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Taq DNA Polymerase


Taq DNA polymerase is purified from E. coli. expressing a cloned Thermus aquaticus DNA polymerase gene. Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5’→3’ DNA polymerase activity and a 5’→3’ exonuclease activity. In PCR, Taq DNA polymerase has an extension speed at 1-2 kb/min.

The enzyme is available in 1,000 and 5,000 unit sizes at a concentration of 5 U/µL. The enzyme is supplied with a 10× Reaction Buffer.


  • Robust performance
  • Leaves 3'A overhang
  • Stable at storage temperature


  • General PCR.
  • Genomic analysis.
  • TA cloning.

Product Specifications

  • Storage Buffer: 20 mM Tris-HCl (pH 8.0 at 25oC), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.5% (v/v) NP-40, and 50% (v/v) glycerol.
  •  Unit Definition: One unit is defined as the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
  • Unit Assay Conditions: 25 mM TAPS (pH 9.3), 50 mM KCl, 2 mM MgCl2, 1 mM DTT, 0.5 mg/ml activated salmon sperm DNA, 0.2 mM dATP, dCTP, dGTP, dTTP.
  • 10× Reaction Buffer: 200 mM Tris-HCl (pH 8.8), 100 mM KCl, 25 mM MgCl2, and other components.


To Order

Buy Cat. No. Product Name Unit Size Price
D006-1 Taq DNA Polymerase 1000U $75
D006-2 Taq DNA Polymerase 5000U $300


Protocol (PDF): D006
MSDS (PDF): msds-D006