The 1 kb plus DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 300 bp to 10 kb. The ladder contains a total of twelve bands: 300, 500, 800, 1000, 1500, 2000, 3000, 4000, 5000, 6000, 8000, 10000bp. The 2 kb band has a concentration of 100 ng/5 µL with increased intensity to provide internal orientation. The other bands have a concentration of 50 ng/5 µL. The 1 kb plus DNA Ladder is provided in 1X loading buffer.
The ladders are generated from PCR and restriction enzyme digestion of double stranded DNA. The DNA is purified by phenol extraction, and equilibrated to 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.
The Ready-to-Use DNA Ladders are supplied in a ready-to- load format. For agarose gel electrophoresis, load ~1 µL of DNA ladder per mm lane.