The 100bp plus DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 100 bp to 5000 bp. The ladder contains a total of fourteen bands: 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1500, 2000, 3000, 5000bp. The 500 bp band has a concentration of 100 ng/5 µL with increased intensity to provide internal orientation. The other bands have a concentration of 50 ng/5 µL. The 100 bp plus DNA Ladder is provided in 1X loading buffer.
The ladders are generated from PCR and restriction enzyme digestion of double stranded DNA. The DNA is purified by phenol extraction, and equilibrated to 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.
The Ready-to-Use DNA Ladders are supplied in a ready-to- load format. For agarose gel electrophoresis, load ~1 µL of DNA ladder per mm lane.