Protein Detection and Quantitation

Protein gel stain and Western Blot are important tool in proteomics (Figure 1). ABP Biosciences offers two distinct protein gel stains: 1) Coomassie® Blue Fast Stain, a ready-to-use product that does not require methanol or acetic acid fixatives or destains; 2) eLuminol™ Protein Gel Stain, a highly-sensitive fluorescent stain designed to detect proteins in SDS polyacrylamide gels. In addition, ABP Biosciences also offers a phosphoprotein selective stain: Phospho-Tag™ Phosphoprotein Gel Stain. ABP Biosciences’ ECL Western Blotting Detection Kit provides highly sensitive detection on immunoblots. Besides, ABP Biosciences also offers a protein assay kit: BCA Protein Assay Kit.

Figure 1. Protein gel analysis workflow

Protein Gel Stain Selection Guide

Figure 2. Gel stain results with Coomassie® Blue Fast Stain (left), eLuminol™ Protein Gel Stain (middle), and Phos-Tag™ Phosphoprotein Gel Stain, followed by eLuminol™ total protein stain (right). Two-fold serial dilutions (from left to right) of protein marker were separated via SDS-PAGE and then stained with above stains.