Protein gel stain and Western Blot are important tool in proteomics (Figure 1). ABP Biosciences offers two distinct protein gel stains: 1) Coomassie® Blue Fast Stain, a ready-to-use product that does not require methanol or acetic acid fixatives or destains; 2) eLuminol™ Protein Gel Stain, a highly-sensitive fluorescent stain designed to detect proteins in SDS polyacrylamide gels. In addition, ABP Biosciences also offers a phosphoprotein selective stain: Phospho-Tag™ Phosphoprotein Gel Stain. ABP Biosciences’ ECL Western Blotting Detection Kit provides highly sensitive detection on immunoblots. Besides, ABP Biosciences also offers a protein assay kit: BCA Protein Assay Kit.
Figure 1. Protein gel analysis workflow
Protein Gel Stain Selection Guide
Coomassie® Blue Fast Stain | eLuminol™ Protein Gel Stain | Phospho-Tag™ Phosphoprotein Gel Stain | |
Sensitivity | 20 ng | 0.5 ng | 1 ng |
Ease of use | Ready to use | Supplied as stock solution | Ready to use |
Time required |
2 hr (standard) 20 min (microwave) |
90 min (standard) 30 min (microwave) |
4 hr (standard) 2 hr (microwave) |
Detection method | colorimetric | fluorescent | fluorescent |
MS spec. compatible | Yes | Yes | Yes |
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Figure 2. Gel stain results with Coomassie® Blue Fast Stain (left), eLuminol™ Protein Gel Stain (middle), and Phospho-Tag™ Phosphoprotein Gel Stain, followed by eLuminol™ total protein stain (right). Two-fold serial dilutions (from left to right) of protein marker were separated via SDS-PAGE and then stained with above stains.
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