LipoFectMax™ Transfection Reagent is a lipid-based transfection reagent that forms a complex with DNA or RNA, and transports the complex into a variety of adherent and suspension cell lines. LipoFectMax™ Transfection Reagent has been tested to work the same efficiency as Lipofectamine® 2000 Reagent, and used for the transfection of both DNA and RNA into eukaryotic cells even in the presence of serum.
- Superior transfection efficiency for a broad range of cell lines
- Does not require removal of serum or culture medium
- Does not require washing or changing of medium after transfection
- Low cytotoxicity
Figure 1. Assessment of LipoFectMax™ transfection efficiencies using GFP visualization. 8 commonly-used cell lines were transfected with a GFP-expressing plasmid using LipoFectMax™ transfection reagent. Cells were visualized 48 hours post-transfection under fluorescence microscopy (GFP filter).
Figure 3. Assessment of LipoFectMax™ transfection efficiencies for siRNA. Hela cell line was transfected with either a GFP cDNA (Left), or co-tranfected with GFP cDNA and an anti-GFP siRNA (right). Transfection efficiencies were assessed by GFP signal.
Figure 4. Comparison of LipoFectMax™ and Lipofectamine® 2000 in plasmid transfection on mouse neuronal cells. Mouse neuronal cells were transfected with a GFP-expressing plasmid using either LipoFectMax™ transfection reagent (left), or Lipofectamine® 2000 (right). Cells were visualized 48 hours post-transfection under fluorescence microscopy (GFP filter).