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Biotin-14-dCTP

Introduction

Biotin-14-dCTP is used for DNA biotinylation, an excellent, and sometimes superior alternative to conventional radioisotopic labeling. Advantages of using biotinylated DNA probes include:

• Convenience and safety—probe preparation generates no radioactive waste
• Stability—probes remain active longer than isotopically labeled probes
• Versatility—biotinylated DNA can be used for immobilization and capture procedures via streptavidin binding

Biotin-14-dCTP is a dCTP analog with biotin attached at the N4-position of the pyrimidine base by a 14-atom linker. This nucleotide can be efficiently incorporated into DNA by nick translation in the presence of dATP, dGTP, and dTTP, labeling DNA with terminal deoxynucleotide transferase, or random primer synthesis. The amount of material provided is sufficient for labeling up to 50 µg of DNA.
 

Specifications:

Concentration: 0.4 mM in 100 mM Tris-HCl (pH 7.5), 0.1 mM EDTA.
Storage: Store at -20°C
Product size: 50 nmol

 

To order

Buy Catalog # Product Name Ex/Em (nm) Unit Size List Price
C430 Biotin-14-dCTP 50 nmol $350

Document

Protocol (PDF): C430  
MSDS (PDF):  MSDS-C430  

References:

Comparison of detection and signal amplification methods for DNA microarrays.Comparison of detection and signal amplification methods for DNA microarrays.
Vora GJ, Meador CE, Anderson GP, Taitt CR,
Mol Cell Probes (2008) 22:294-300

Differential incorporation of biotinylated nucleotides by terminal deoxynucleotidyl transferase.Differential incorporation of biotinylated nucleotides by terminal deoxynucleotidyl transferase.
Flickinger JL, Gebeyehu G, Buchman G, Haces A, Rashtchian A,
Nucleic Acids Res (1992) 20:2382-2382

Novel biotinylated nucleotide–analogs for labeling and colorimetric detection of DNA.Novel biotinylated nucleotide–analogs for labeling and colorimetric detection of DNA.
Gebeyehu G, Rao PY, SooChan P, Simms DA, Klevan L
Nucleic Acids Res (1987) 15:4513-4534