Nucleic Acid Quantitation

ABP Biosciences iQuant™ DNA or RNA Assay Kits allow precise quantitation of DNA or RNA samples across a wide range of concentrations. Our iQuant™ assay kits are ideal for quantitating DNA or RNA samples in Next Gen Sequencing applications. Unlike absorbance-based nucleic acid quantitation, fluorescence-based DNA or RNA quantitation is highly sensitive and selective for DNA or RNA, and provides more accurate DNA or RNA concentration in the presence of common contaminants, including free nucleotides, protein, detergents and salts.

Features

DNA quantitation of purified DNA samples is a standard nucleic acid research workflow, providing confidence for downstream applications such as Next Gen Sequence, PCR, Cloning, and DNA transfections (Figure 1).

Figure 1. Nucleic acid research workflow

ABP Biosciences iQuant™ 1X dsDNA HS assay kit provides a very simple workflow (Figure 2).

Figure 2. iQuant™ 1X dsDNA HS assay workflow

In addition, ABP Biosciences iQuant™ DNA/RNA Assay Kits provide higher sensitivity than absorbance-based quantitation methods (Figure 3).

                            
Figure 3. Improved sensitivity with iQuant™ DNA/RNA Assay Kits

Further, iQuant™ DNA Assay Kits exhibit excellent selectivity for DNA, even in the presence of an equal mass of RNA (Figure 4).

Figure 4. Selectivity for dsDNA using iQuant™ dsDNA HS Assay Kit in the presence of an equal mass of RNA.

iQuant™ DNA Quantitation Kits show superior accuracy for detecting dsDNA compared with absorbance-based quantitation method, especially at relatively low DNA concentrations (Figure 5).

Figure 5. Comparison of accuracy of DNA quantitation between the iQuant™ dsDNA HS Assay Kit and absorbance-based quantitation method.