ABP Biosciences iQuant™ DNA or RNA Assay Kits allow precise quantitation of DNA or RNA samples across a wide range of concentrations. Our new iQuant™ NGS dsDNA assay kits are ideal for quantitating DNA samples in Next Gen Sequencing applications. Unlike absorbance-based nucleic acid quantitation, fluorescence-based DNA or RNA quantitation is highly sensitive and selective for DNA or RNA, and provides more accurate DNA or RNA concentration in the presence of common contaminants, including free nucleotides, protein, detergents and salts.
- Higher sensitivity: 100 times more sensitive than absorbance assay;
- Broad dynamic range: over four orders of magnitude;
- More accuracy: unaffected by the presence of proteins, free nucleotides;
- Easy to use: mix-and-read format;
- Instrument compatibility: assay can be performed using a fluorescence plate reader, or a fluorometer.
DNA quantitation of purified DNA samples is a standard nucleic acid research workflow, providing confidence for downstream applications such as Next Gen Sequence, PCR, Cloning, and DNA transfections (Figure 1).
Figure 1. Nucleic acid research workflow
ABP Biosciences iQuant™ NGS dsDNA assay kits provide a very simple workflow (Figure 2).
Figure 2. iQuant™ NGS dsDNA assay workflow
In addition, ABP Biosciences iQuant™ DNA/RNA Assay Kits provide higher sensitivity than absorbance-based quantitation methods (Figure 3).
Figure 3. Improved sensitivity with iQuant™ DNA/RNA Assay Kits
Further, iQuant™ DNA Assay Kits exhibit excellent selectivity for DNA, even in the presence of an equal mass of RNA (Figure 4).
Figure 4. Selectivity for dsDNA using iQuant™ dsDNA HS Assay Kit in the presence of an equal mass of RNA.
iQuant™ DNA Quantitation Kits show superior accuracy for detecting dsDNA compared with absorbance-based quantitation method, especially at relatively low DNA concentrations (Figure 5).
Figure 5. Comparison of accuracy of DNA quantitation between the iQuant™ dsDNA HS Assay Kit and absorbance-based quantitation method.
iQuant™ DNA/RNA Assay Kits Selection Guide
|Target||Initial sample Concentration||Application|
|iQuant™ dsDNA HS Assay Kit||dsDNA||10 pg/µL – 100 ng/µL||Useful for quantitation of PCR products, viral DNA, and samples for subcloning and Next Gen Sequencing|
|iQuant™ 1X dsDNA HS Assay Kit||dsDNA||10 pg/µL – 100 ng/µL||Useful for quantitation of DNA samples for Next Gen Sequencing|
|iQuant™ dsDNA BR Assay Kit||dsDNA||100 pg/µL – 1,000 ng/µL||Useful for quantitation of genomic and miniprep DNA samples|
|iQuant™ ssDNA Assay Kit||ssDNA||20 pg/µL – 200 ng/µL||Useful for quantitation of oligo, primers, denatured DNA, PCR products|
|iQuant™ RNA HS Assay Kit||RNA||100 pg/µL – 100 ng/µL||Useful for quantitation of samples for microarray, RT-PCR, and Northern blot procedures|
|iQuant™ RNA BR Assay Kit||RNA||400 pg/µL – 1,000 ng/µL||Useful for quantitation of samples for microarray, RT-PCR, and Northern blot procedures|
|iQuant™ microRNA Assay Kit||small RNA||0.025 ng/µL – 150 ng/µL||Useful for quantitation of microRNA, siRNA.|
- Nucleic Acid Gel Stains
- Nucleic Acid Quantitation
- Labeled Nucleotides
- Protein Detection and Quantitation
- Cell Structure Probes
- Secondary Antibody and Streptavidin
- Cell Proliferation & Viability Assay
- Cell Apoptosis Assay
- Andy Fluor™ Dyes
- Ion Indicators
- Transfection Reagent
- Luciferase Assay Kit
- ECL Western Blot Reagent