iQuant™ RiboGreen RNA Assay Kit

The iQuant™ RiboGreen RNA Assay Kit is one of the most sensitive detection kits for quantitation of RNA in solution, with linear fluorescence detection in the range of 1-200 ng of RNA. The iQuant™ RiboGreen RNA Reagent enables quantitation of as little as 1 ng/mL RNA (200 pg RNA in a 200 µL assay volume) with a fluorescence microplate reader. The linear range of the iQuant™ RiboGreen RNA Reagent extends over three orders of magnitude in RNA concentration (1 ng/mL to 1 µg/mL)  using two dye concentrations (Figure 1). The high-range assay allows quantitation of 20 ng/mL to 1 µg/mL RNA, and the low-range assay allows quantitation of 1 ng/mL to 50 ng/mL RNA. The assay kit contains RiboGreen RNA Reagent, assay buffer, and RNA standard. The assay is well tolerated to common contaminants such as proteins, salts, nucleotides, urea, ethanol, chloroform, detergents, and agarose. 
 

Figure 1. Left: The high-range assay, the iQuant™ RiboGreen RNA Reagent was diluted 200-fold. Right: The low-range assay, the iQuant™ RiboGreen RNA Reagent was diluted 2000-fold.

Technical Note: Encapsulation Efficiency Assay for RNA-LNPs

The iQuant™ RiboGreen RNA Assay Kit can be used to measure encapsulation efficiency and RNA concentration for RNA-LNPs. The detail protocol can be downloaded from here.

Specifications:

Applications

RNA quatitation in solution.