The iQuant™ RNA HS Assay Kit is designed specifically for an easy and accurate quantitation of RNA. The kit is highly selective for RNA in the present of DNA, and offers advantages in stability, linear dynamic range, and sensitivity over other traditional of RNA quantitation. The kit contains concentrated quantitation reagent, dilution buffer, and pre-diluted RNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the fluorescence using fluorescence plate reader or Fluorometer (Figure 1). The assay is well tolerated to common contaminants such as proteins, salts, solvents, detergents, or free nucleotides. The assay can be adapted for use in microplates, tubes or cuvettes.
Figure 1. Left: Quantitation of ribosomal RNA with iQuant™ RNA HS Assay Kit using fluorescence plate reader. Right: Quantitation of ribosomal RNA with iQuant™ RNA HS Assay Kit using Qubit® Fluorometer.
|Platform:||Fluorescence plate reader or fluorometer|
|Linear Range:||5-100 ng RNA|
RNA quatitation in solution (Figure 2).
Figure 2. iQuant™ RNA HS assay workflow