Transfection Reagent

Transfection is the process by which nucleic acids are introduced into mammalian cells. When selecting a transfection reagent, consider the type of nucleic acids (DNA, or RNA) you wish to deliver and the type of cells you want to transfect. 
 

Transfection Reagent Selection Guide

Cationic Lipid based Transfection Reagent
 

ABP Biosciences developed novel cationic lipid based transfection reagents used for DNA/siRNA transfection. LipoFectMax™ Transfection Reagent has good transfection efficiency for most eukaryotic cells (attached or suspended). LipoFectMax™ 3000 Transfection Reagent has higher transfection efficiency for some difficult-to-transfect cells, primary cells, and neuronal cells.

Figure 1. Comparison of LipoFectMax™ and LipoFectMax™ 3000 with Lipofectamine® 2000 and Lipofectamine® 3000 transfection efficiencies. Expi293F, CHO, A549, NIH-3T3, COS-7, HepG2 cells were transfected with a GFP-expressing plasmid using LipoFectMax™, LipoFectMax™ 3000, Lipofectamine® 2000 and Lipofectamine® 3000 transfection reagent, respectively. Cells were evaluated for transfection efficiencies after 48 hours post-transfection.

Figure 2. The transfection efficiencies of LipoFectMax™ and LipoFectMax™ 3000 in Expi293F, and CHO suspension cells both are over 95%, ideal for protein expression.

Biodegradable Cationic Polymer based Transfection Reagent
 

ABP Biosciences developed a novel biodegradable cationic polymer based transfection reagents used for DNA transfection. PolyFect™ Transfection Reagent is suitable for a variety of adherent and suspension cell lines.

Figure 3. Comparison of PolyFect™ and Lipofectamine® 2000 transfection efficiencies. Expi293F, CHO, A549, NIH-3T3, COS-7, HepG2 cells were transfected with a GFP-expressing plasmid using either PolyFect™ transfection reagent (yellow), or Lipofectamine® 2000 (blue). Cells were evaluated for transfection efficiencies after 48 hours post-transfection.

Figure 4. The transfection efficiencies of PolyFect™ in Expi293F, and CHO suspension cells are over 95%, ideal for protein expression.