Transfection is the process by which nucleic acids are introduced into mammalian cells. When selecting a transfection reagent, consider the type of nucleic acids (DNA, or RNA) you wish to deliver and the type of cells you want to transfect.
Transfection Reagent Selection Guide
|Cat No.||Product Name||Application||Cell Type||Replacement for|
|FP310||LipoFectMax™ Transfection Reagent||DNA, RNA transfection||A variety of eukaryotic cells||Lipofectamine™ 2000|
|FP318||LipoFectMax™ 3000 Transfection Reagent||DNA, RNA transfection||The widest selection of hard-to-transfect and common cells||Lipofectamine™ 3000|
|FP330||PolyFect™ Transfection Reagent||DNA transfection||A variety of eukaryotic cells||PolyJet™|
|FP332||PolyFectRNA™ Transfection Reagent||siRNA or miRNA transfection||A variety of eukaryotic cells||Lipofectamine™ RNAiMAX|
|FP315||Fect293™ Transfection Reagent||Protein expression||Suspension 293 cells||ExpiFectamine™ 293|
|FP316||FectCHO™ Transfection Reagent||Protein expression||Suspension CHO cells||ExpiFectamine™ CHO|
|FP317||PolyFectVIR™ Transfection Reagent||Virus production||HEK-293 cells||FectoVIR®-AAV, PEIpro®|
Cationic Lipid based Transfection Reagent
ABP Biosciences developed novel cationic lipid based transfection reagents used for DNA/siRNA transfection. LipoFectMax™ Transfection Reagent has good transfection efficiency for most eukaryotic cells (attached or suspended). LipoFectMax™ 3000 Transfection Reagent has higher transfection efficiency for some difficult-to-transfect cells, primary cells, and neuronal cells.
Figure 1. Comparison of LipoFectMax™ and LipoFectMax™ 3000 with Lipofectamine® 2000 and Lipofectamine® 3000 transfection efficiencies. Expi293F, CHO, A549, NIH-3T3, COS-7, HepG2 cells were transfected with a GFP-expressing plasmid using LipoFectMax™, LipoFectMax™ 3000, Lipofectamine® 2000 and Lipofectamine® 3000 transfection reagent, respectively. Cells were evaluated for transfection efficiencies after 48 hours post-transfection.
Figure 2. The transfection efficiencies of LipoFectMax™ and LipoFectMax™ 3000 in Expi293F, and CHO suspension cells both are over 95%, ideal for protein expression.
Biodegradable Cationic Polymer based Transfection Reagent
ABP Biosciences developed a novel biodegradable cationic polymer based transfection reagents used for DNA transfection. PolyFect™ Transfection Reagent is suitable for a variety of adherent and suspension cell lines.
Figure 3. Comparison of PolyFect™ and Lipofectamine® 2000 transfection efficiencies. Expi293F, CHO, A549, NIH-3T3, COS-7, HepG2 cells were transfected with a GFP-expressing plasmid using either PolyFect™ transfection reagent (yellow), or Lipofectamine® 2000 (blue). Cells were evaluated for transfection efficiencies after 48 hours post-transfection.
Figure 4. The transfection efficiencies of PolyFect™ in Expi293F, and CHO suspension cells are over 95%, ideal for protein expression.
- Nucleic Acid Gel Stains
- Nucleic Acid Quantitation
- Labeled Nucleotides
- Protein Detection and Quantitation
- Cell Structure Probes
- Secondary Antibody and Streptavidin
- Cell Proliferation & Viability Assay
- Cell Apoptosis Assay
- Andy Fluor™ Dyes
- Ion Indicators
- Transfection Reagent
- Luciferase Assay Kit
- ECL Western Blot Reagent