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Transfection Reagent

Introduction

Transfection is the process by which nucleic acids are introduced into mammalian cells. When selecting a transfection reagent, consider the type of nucleic acids (DNA, or RNA) you wish to deliver and the type of cells you want to transfect. 
 

Transfection Reagent Selection Guide

 

Cat No. Product Name Application Cell Type Replacement for 
FP310 LipoFectMax™ Transfection Reagent DNA, RNA transfection A variety of eukaryotic cells Lipofectamine™ 2000
FP318 LipoFectMax™ 3000 Transfection Reagent DNA, RNA transfection The widest selection of hard-to-transfect and common cells Lipofectamine™ 3000
FP330 PolyFect™ Transfection Reagent DNA transfection A variety of eukaryotic cells PolyJet™
FP332 PolyFectRNA™ Transfection Reagent siRNA or miRNA transfection A variety of eukaryotic cells Lipofectamine™ RNAiMAX
FP315 Fect293™ Transfection Reagent Protein expression Suspension 293 cells ExpiFectamine™ 293
FP316 FectCHO™ Transfection Reagent Protein expression Suspension CHO cells ExpiFectamine™ CHO
FP317 PolyFectVIR™ Transfection Reagent Virus production HEK-293 cells FectoVIR®-AAV, PEIpro®


Cationic Lipid based Transfection Reagent
 

ABP Biosciences developed novel cationic lipid based transfection reagents used for DNA/siRNA transfection. LipoFectMax™ Transfection Reagent has good transfection efficiency for most eukaryotic cells (attached or suspended). LipoFectMax™ 3000 Transfection Reagent has higher transfection efficiency for some difficult-to-transfect cells, primary cells, and neuronal cells.

 

Figure 1. Comparison of LipoFectMax™ and LipoFectMax™ 3000 with Lipofectamine® 2000 and Lipofectamine® 3000 transfection efficiencies. Expi293F, CHO, A549, NIH-3T3, COS-7, HepG2 cells were transfected with a GFP-expressing plasmid using LipoFectMax™, LipoFectMax™ 3000, Lipofectamine® 2000 and Lipofectamine® 3000 transfection reagent, respectively. Cells were evaluated for transfection efficiencies after 48 hours post-transfection.

Figure 2. The transfection efficiencies of LipoFectMax™ and LipoFectMax™ 3000 in Expi293F, and CHO suspension cells both are over 95%, ideal for protein expression.


Biodegradable Cationic Polymer based Transfection Reagent
 

ABP Biosciences developed a novel biodegradable cationic polymer based transfection reagents used for DNA transfection. PolyFect™ Transfection Reagent is suitable for a variety of adherent and suspension cell lines.


Figure 3. Comparison of PolyFect™ and Lipofectamine® 2000 transfection efficiencies. Expi293F, CHO, A549, NIH-3T3, COS-7, HepG2 cells were transfected with a GFP-expressing plasmid using either PolyFect™ transfection reagent (yellow), or Lipofectamine® 2000 (blue). Cells were evaluated for transfection efficiencies after 48 hours post-transfection.


Figure 4. The transfection efficiencies of PolyFect™ in Expi293F, and CHO suspension cells are over 95%, ideal for protein expression.

To order

Buy Cat.No. Product Name Unit Price
FP310 LipoFectMax™ Transfection Reagent 1 mL $200
FP311 3 mL $400
FP318 LipoFectMax™ 3000 Transfection Reagent 0.75 mL $300
FP319 1.5 mL $450
FP330 PolyFect™ Transfection Reagent 1 mL $160
FP331 3 mL $450
FP332 PolyFectRNA™ Transfection Reagent 1 mL $250
FP333 3 mL $550
FP315 Fect293™ Transfection Reagent 1 mL $220
FP316 FectCHO™ Transfection Reagent 1 mL $220
FP317-1 PolyFectVIR™ Transfection Reagent 1 mL $200
FP317-2 10 mL $1400
FP317-3 100 mL $10000