Introduction
FectCHO™ Transfection Reagent is a biodegradable polymer based transfection reagent that forms a complex with DNA, and transports the complex into a variety of adherent and suspension cell lines. A remarkable feature of the reagent is the rapid and complete degradation of polymer after transfection complex endocytosis, leading to much less cytotoxicity.
Features
- Superior transfection efficiency in both suspension and adherent CHO cells
- Does not require removal of serum or culture medium
- Does not require washing or changing of medium after transfection
- Low cytotoxicity
Figure 1. Comparison of FecCHO™ and Lipofectamine® 2000 transfection efficiencies using GFP visualization. CHO cells were transfected with a GFP-expressing plasmid using either FectCHO™ transfection reagent (left), or Lipofectamine® 2000 (right). Cells were visualized 48 hours post-transfection under fluorescence microscopy (GFP filter).
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