Introduction
QSY7 quencher succinimidyl ester is a nonfluorescent acceptor dye for preparation of peptide and oligonucleotide FRET probes.
Features
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Fluorescence quantum yields <0.001 in aqueous solution;
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Absorption spectra of the conjugates are insensitive to pH between 4 and 10;
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Efficient quenching of the fluorescence emission of donor dyes including blue-fluorescent coumarins, green- or orange-fluorescent dyes, and red-fluorescent dye conjugates;
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Quenching of most green and red fluorophores is more effective at far greater distances than is possible with dabcyl quenchers.
Specifications
Reactivity:
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Primary amine
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Abs maximum:
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560 nm
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Extinction coefficient:
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90,000 cm-1M-1
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Solubility:
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DMSO, DMF
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Molecular Formula:
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C43H39ClN4O7S
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Molecular Weight:
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791.32
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CAS Number:
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304014-12-8
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Storage Conditions:
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-20 ℃, protect from light
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Shipping Condition:
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Room Temperature
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Applications
FRET probe.
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Documents
References:
A novel approach to the analysis of specificity, clonality, and frequency of HIV-specific T cell responses reveals a potential mechanism for control of viral escape.
Douek DC, Betts MR, Brenchley JM, Hill BJ, Ambrozak DR, Ngai KL, Karandikar NJ, Casazza JP, Koup RA
J Immunol (2002) 168:3099-3104
Actin filament uncapping localizes to ruffling lamellae and rocketing vesicles.
Allen PG
Nat Cell Biol (2003) 5:972-979
Beta-secretase cleavage at amino acid residue 34 in the amyloid beta peptide is dependent upon gamma-secretase activity.
Shi XP, Tugusheva K, Bruce JE, Lucka A, Wu GX, Chen-Dodson E, Price E, Li Y, Xu M, Huang Q, Sardana MK, Hazuda DJ
J Biol Chem (2003) 278:21286-21294