Introduction
The primary aliphatic amine of 4'-(aminomethyl)fluorescein can be reversibly coupled to aldehydes and ketones to form a Schiff base – which can be reduced to a stable amine derivative by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNBH3) to form new probes. Carboxylic acids of proteins and other water-soluble biopolymers can be coupled to this molecule in aqueous solution using water-soluble carbodiimides such as EDAC.
Specifications
Label:
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Fluorescein
|
|
Ex/Em:
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494/520 nm
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Detection Method:
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Fluorescent
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Solubility:
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DMSO, DMF
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Molecular Formula:
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C21H16ClNO5
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Molecular Weight:
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397.81
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CAS Number:
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91539-64-9
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Storage Conditions:
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-20 ℃, protect from light
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Shipping Condition:
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Room Temperature
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Applications
Fluorescent labeling
|
Documents
References:
Kim AJ, Manoharan VN, Crocker JC
J Am Chem Soc (2005) 127:1592-1593
Product usage:
Shipchandler MT, Fino JR, Klein LD, Kirkemo CL
Anal Biochem (1987) 162:89-101
Product usage:
Clackson T, Yang W, Rozamus LW, Hatada M, Amara JF, Rollins CT, Stevenson LF, Magari SR, Wood SA, Courage NL, Lu X, Cerasoli F, Gilman M, Holt DA
Proc Natl Acad Sci U S A (1998) 95:10437-10442