EasyProbe™ DAPI Fixed Cell Stain

EasyProbes™ DAPI is a commonly used nuclear counterstain for fixed cells that emits blue fluorescence upon binding to the minor groove of dsDNA. Our EasyProbes™ DAPI Fixed Cell Stain is formulated in a ready-to-use solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. DAPI is widely used for nuclear and chromosome counterstain, showing a distinct banding pattern in chromosomes.

Features

Suggestions for use

• In most cases 2 drops/ml and an incubation of 15 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases simply add more, or fewer drops until the optimal staining intensity is obtained.
• EasyProbes™ DAPI Fixed Cell Stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm. It is detected through a blue/cyan filter, such as a DAPI filter set.

Specifications:
Excitation/Emission:	358/460 nm
Shipping Condition:	Ambient
Storage Conditions:	4ºC, protect from light
Unit Size:	10 mL