Ethidium Homodimer-1 (EthD-1) is a high affinity fluorescent nucleic acid stain that is weakly fluorescent until bound to DNA and emits red fluorescence (excitation/emission maxima ~528/617). The EthD-1 is highly positively charged and cell-impermeant, and widely used as cell viability indicator.
|Storage Conditions:||-20ºC, protect from light|
Multiplex dsDNA Fragment Sizing Using Dimeric Intercalation Dyes and Capillary Array Electrophoresis: Ionic Effects on the Stability and Electrophoretic Mobility of DNA-Dye Complexes.
Clark SM, Mathies RA
Anal Chem (1997) 69:1355-1355
Reliability of Calcein Acetoxy Methyl Ester and Ethidium Homodimer or Propidium Iodide for Viability Assessment of Microbes.Reliability of Calcein Acetoxy Methyl Ester and Ethidium Homodimer or Propidium Iodide for Viability Assessment of Microbes.
Kaneshiro ES, et al.
J Microbiol Methods (1993) 17:1-1
Assessment of Boar Sperm Viability Using a Combination of Two Fluorophores.Assessment of Boar Sperm Viability Using a Combination of Two Fluorophores.
Althouse GC, Hopkins SM
Theriogenology (1995) 43:595-595
Frequently Asked Questions (FAQs)
I am doing a Live/Dead assay using Calcein, AM, for live cells and ethidium homodimer-1 for dead cells. Can I fix the cells after labeling and retain the staining?
This is not recommended. Neither Calcein nor ethidium homodimer-1 bind to any cellular components upon fixation. There is no guarantee that the dyes will be retained upon fixation or any subsequent wash steps. We recommend scoring for live and dead cells as soon as possible after staining.
- EasyProbe™ DAPI Fixed Cell Stain
- EasyProbe™ Hoechst 33342 Live Cell Stain
- EasyProbe™ Propidium Iodide Dead Cell Stain
- EasyProbe™ Green 488 Live Cell Stain
- EasyProbe™ Green 488 Dead Cell Stain
- EMA (Ethidium monoazide)
- EthD-1 (Ethidium Homodimer-1)
- NucGreen™ live-cell nucleic acid stain
- NucGreen™ dead-cell nucleic acid stain
- NucRed™ live-cell nucleic acid stain
- Acridine orange
- DRAQ5™ Fluorescent Probe