Introduction
Ethidium monoazide is a fluorescent photoafinity label nucleic acid stain, after photolysis, which binds covalently to nucleic acids both in solution and in cells that have compromised membranes. Ethidium monoazide bromide is relatively impermeant to live cells, it selectively labels DNA in dead cells in a mixed population of live and dead cells. The fluorescence of ethidium monoazide is weak, but the intensity increases ~15-fold upon binding to DNA with excitation/emission maxima of ~504/600 nm.
| Specifications: |
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| Excitation/Emission: | 504/600 nm | |
| Shipping Condition: | Ambient | |
| Storage Conditions: | -20ºC, protect from light | |
| Molecular Formula: | C21H18BrN5 | |
| Molecular Weight: | 420.31 | |
| CAS Number: | 58880-05-0 | |
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To Order
Documents
| Protocol (PDF): | C009 |
| MSDS (PDF): | MSDS-C009 |
| COA (PDF): | C009 |
Reference:
Shiina M, Rehermann B,
Methods Mol Biol (2009) 510:415-426
Methods Mol Biol (2009) 510:415-426
Fluorescent Imaging of Cryptosporidium Using a Cooled Charge Couple Device (CCD).
Campbell AT, et al.
J Microbiol Methods (1992) 16:169-169
J Microbiol Methods (1992) 16:169-169
Latz E, Schoenemeyer A, Visintin A, Fitzgerald KA, Monks BG, Knetter CF, Lien E, Nilsen NJ, Espevik T, Golenbock DT
Nat Immunol (2004) 5:190-198
Nat Immunol (2004) 5:190-198
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