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Phospho-Tag™ Phosphoprotein Gel Stain

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Introduction

Phospho-Tag™ Phosphoprotein Gel Stain is a high sensitive fluorescent stain designed for selectively detecting phosphoproteins in polyacrylamide gels. This stain contains a phospho-tag™ group, which allows direct, in-gel detection of phosphate groups attached to tyrosine, serine, or threonine residues, without the need for antibodies or radioisotopes. The stain can be used with standard SDS-polyacrylamide gels or with 2-D gels.


Features

  • High sensitivity. Detect as little as 1 ng phosphoprotein.
  • Simple and fast staining.
  • Compatibility with standard laboratory equipment.
  • Wide linear detection range. At least three orders of magnitude.
  • Compatible with downstream analysis: Compatible with MS and sequencing.
  • Stable: Stable at room temperature for 1 year.
  phos-tag_eluminol


Applications

Protein gel stain

To Order

Buy Catalog # Product Name Description Size List Price
P005A Phospho-Tag™ Phosphoprotein Gel Stain Phosphoprotein gel stain 500 mL $180
P005B Phospho-Tag Phosphoprotein Destain Solution  Destain solution 500 mL $50
   

Documents

Protocol (PDF): P005A
MSDS (PDF):  P005A

Reference:

Phosphoserine acidic cluster motifs bind distinct basic regions on the μ subunits of clathrin adaptor protein complexes.

Singh, R., et al. (2018).The Journal of Biological Chemistry doi: 10.1074/jbc.RA118.003080