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Nucleic Acid Quantitation

Introduction

ABP Biosciences iQuant™ DNA or RNA Assay Kits allow precise quantitation of DNA or RNA samples across a wide range of concentrations. Our new iQuant™ NGS dsDNA assay kits are ideal for quantitating DNA samples in Next Gen Sequencing applications. Unlike absorbance-based nucleic acid quantitation, fluorescence-based DNA or RNA quantitation is highly sensitive and selective for DNA or RNA, and provides  more accurate DNA or RNA concentration in the presence of common contaminants, including free nucleotides, protein, detergents and salts.


Features

  • Higher sensitivity: 100 times more sensitive than NanoDrop;
  • Broad dynamic range: over four orders of magnitude;
  • More accuracy: unaffected by the presence of proteins, free nucleotides;
  • Easy to use: mix-and-read format;
  • Instrument compatibility: assay can be performed using a fluorescence plate reader, or a fluorometer such as Qubit®.

 

DNA quantitation of purified DNA samples is a standard nucleic acid research workflow, providing confidence for downstream applications such as Next Gen Sequence, PCR, Cloning, and DNA transfections (Figure 1).


Figure 1. Nucleic acid research workflow

ABP Biosciences iQuant™ NGS dsDNA assay kits provide a very simple workflow (Figure 2).


Figure 2. iQuant™ NGS dsDNA assay workflow

In addition, ABP Biosciences iQuant™ DNA Assay Kits provide higher sensitivity than absorbance-based quantitation methods, such as NanoDrop (Figure 3).

                            
Figure 3. Improved sensitivity with iQuant™ DNA Assay Kits

Further, iQuant™ DNA Assay Kits exhibit excellent selectivity for DNA, even in the presence of an equal mass of RNA (Figure 4).


Figure 4. Selectivity for dsDNA using iQuant™ dsDNA HS Assay Kit in the presence of an equal mass of RNA.

iQuant™ DNA Quantitation Kits show superior accuracy for detecting dsDNA compared with NanoDrop, especially at relatively low DNA concentrations (Figure 5).


Figure 5. Comparison of accuracy of DNA quantitation between the iQuant™ dsDNA HS Assay Kit and NanoDrop.

To Order

iQuant™ Nucleic Acid Quantitation Selection Guide

  Target Initial sample Concentration Application
iQuant™ dsDNA HS Assay Kit dsDNA 10 pg/µL – 100 ng/µL Useful for quantitation of PCR products, viral DNA, and samples for subcloning and Next Gen Sequencing
iQuant™ NGS-HS dsDNA Assay Kit dsDNA 10 pg/µL – 100 ng/µL Useful for quantitation of DNA samples for Next Gen Sequencing
iQuant™ dsDNA BR Assay Kit dsDNA 100 pg/µL – 1,000 ng/µL Useful for quantitation of genomic and miniprep DNA samples
iQuant™ ssDNA Assay Kit ssDNA 20 pg/µL – 200 ng/µL Useful for quantitation of oligo, primers, denatured DNA, PCR products
iQuant™ RNA HS Assay Kit RNA 100 pg/µL – 100 ng/µL Useful for quantitation of samples for microarray, RT-PCR, and Northern blot procedures
iQuant™ RNA BR Assay Kit RNA 400 pg/µL – 1,000 ng/µL Useful for quantitation of samples for microarray, RT-PCR, and Northern blot procedures

 

Buy Cat. No. Product Name Sensitivity Unit Price
N010 iQuant™ dsDNA HS Assay Kit 0.2-100 ng 200 assays $45
N011 1,000 assays $180
N012 iQuant™ dsDNA BR Assay Kit 2-1000 ng 200 assays $45
N013 1000 assays $180
N014 iQuant™ ssDNA Assay Kit 1-200 ng 200 assays $45
N015 1,000 assays $180
N016 iQuant™ RNA HS Assay Kit 5-100 ng 200 assays $45
N017 1,000 assays $180
N018 iQuant™ RNA BR Assay Kit 20-1000 ng 200 assays $45
N019 1,000 assays $180
N020 iQuant™ NGS-HS dsDNA Assay Kit 0.2-100 ng 200 assays $80
N021 500 assays $160
N022 iQuant™ Assay Tubes 500 tubes $60