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Labeled Nucleotides

Introduction

ABP Biosciences offers 5′-aminoallyl dUTP and UTP analogs, as well as conjugates with biotin and various fluorescent dyes, featuring our exceptionally bright and photostable Andy Fluor dyes. Fluorescent and biotin-labeled dUTP and UTP are widely used for enzymatic incorporation into DNA or RNA.


Features

  • Bright signals with low background;
  • Wide variety of fluorophores;
  • Great for many applications, like Fluorescence in situ Hybridization (FISH; Figure 1), TUNEL (Figure 2), and more.​

Figure 1. Fluorescence in situ Hybridization (FISH) of CEP7/MET probes labeled with Andy Fluor dyes. Human Chromosome 7 Enumeration (CEP7) and Human c-Met gene (MET) probes were labeled with Andy Fluor 488-X-dUTP (green) and Andy Fluor 568-X-dUTP (magenta), respectively, using nick translation. The labeled probes were mixed together in hybridization buffers with blocking DNA, and incubated with HT1080 cells on slides at 37°C overnight. The slides was washed and mounted with AntiFade solution containing DAPI (blue), then visualized with an Epi-Fluorescence microscope.

TUNEL-biotin-11-UTP mouse tongue

Figure 2. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-mediated detection of apoptotic cells in mouse tongue tissue using Biotin-11-dUTP. Left: Cells were labeled with Biotin-11-dUTP, followed by detection with AndyFluor™ 488-streptavidin (green). Nuclei were also counterstained with DAPI (blue). Right: Cells were labeled with Biotin-11-dUTP, followed by detection with horse radish peroxidase (HRP).

To Order

Labeled Nucleotides

Buy Cat. No. Product Name Ex/Em(nm) Unit Size Price
C401 5-Aminoallyl-dUTP, 10 mM in TE buffer 100 µL $120
C402 5-Aminoallyl-UTP, 10 mM in TE buffer 100 µL $110
C403 Biotin-11-dUTP, 1 mM in TE buffer 50 µL $180
C404 Biotin-11-UTP, 1 mM in TE buffer 50 µL $180
C430 Biotin-14-dCTP, 0.4 mM 50 nmol $350
C405 5-FAM-dUTP, 1 mM in TE buffer 495/518 25 µL $140
C406 5-FAM-X-dUTP, 1 mM in TE buffer 495/518 25 µL $180
C407 5-FAM-UTP, 1 mM in TE buffer 495/518 25 µL $140
C408 5-FAM-X-UTP, 1 mM in TE buffer 495/518 25 µL $180
C409 Andy Fluor 488-X-dUTP, 1 mM in TE buffer 500/525 25 µL $280
C410 Andy Fluor 488-X-UTP, 1 mM in TE buffer 500/525 25 µL $280
C411 Andy Fluor 555-X-dUTP, 1 mM in TE buffer 553/565 25 µL $280
C412 Andy Fluor 555-X-UTP, 1 mM in TE buffer 553/565 25 µL $280
C413 Andy Fluor 568-X-dUTP, 1 mM in TE buffer 578/602 25 µL $280
C414 Andy Fluor 568-X-UTP, 1 mM in TE buffer 578/602 25 µL $280
C415 Andy Fluor 594-X-dUTP, 1 mM in TE buffer 590/615 25 µL $280
C416 Andy Fluor 594-X-UTP, 1 mM in TE buffer 590/615 25 µL $280
C417 Andy Fluor 647-X-dUTP, 1 mM in TE buffer 650/665 25 µL $280
C418 Andy Fluor 647-X-UTP, 1 mM in TE buffer 650/665 25 µL $280
C419 Cy3-X-dUTP, 1 mM in TE buffer 553/565 25 µL $240
C420 Cy3-X-UTP, 1 mM in TE buffer 553/565 25 µL $240
C421 Cy5-X-dUTP, 1 mM in TE buffer 650/665 25 µL $240
C422 Cy5-X-UTP, 1 mM in TE buffer 650/665 25 µL $240