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Andy Fluor™ and Cy® Dyes

Introduction

Fluorescent labeling of biomolecules enables researchers to detect specific components of complex biological assemblies with exquisite sensitivity in applications ranging from imaging and flow cytometry, to Western blots. ABP Biosciences offers a full spectrum of Andy Fluor™ Dyes for labeling antibodies, proteins, peptides, ligands, synthetic oligonucleotides and other biomolecules for use with immunochemistry, fluorescence in situ hybridization (FISH), cell tracing, receptor labeling, and cytochemistry applications as well as probing biological structure, function and interactions.
ABP Biosciences offers a series of superior bright, highly photo-stable and excellent water-soluble Andy Fluor™ dyes spanning the visible and near-infrared (IR) spectrum for labeling biomolecules, especially proteins and nucleic acids. 
Our Andy Fluor™ antibody conjugates show brighter fluorescence signal than other fluorophore conjugates (Figure 1, and 2). Using our Andy Fluor™ dyes, you will get the brightest conjugates available for imaging, flow cytometry, and other fluorescence-based applications. Our Andy Fluor™ dyes and conjugates also show good photostability, allowing more time for image capture (Figure 3). In addition to superior brightness and good photostability, ABP Biosciences offers a wide selection of Andy Fluor™ dyes across the spectrum for multiplexing and multicolor detection.
 

Features

  • Brightness—Andy Fluor™ has superior brightness
  • Photostability—Andy Fluor™ has good photostability
  • Multiplexing—Andy Fluor™ across spectrum from UV to near-IR
  • pH sensitivity—Andy Fluor™ is insensitive to pH change
  • Water solubility—Andy Fluor™ has good water solubility

Product information

Andy Fluor Dye Selection Guide

Andy Fluor™ Dye Ex/Em (nm) Laser Type Replacement for Advantages
Andy Fluor™ 350 350/440 UV AMCA, Alexa Fluor 350, DyLight 350 Highly fluorescent; Highly water-soluble and pH insensitive
Andy Fluor™ 405M 405/450 405 nm laser Pacific Blue, BD Horizon V450, eFluor 450 Brighter than Pacific Blue; Less spill-over in the green channel
Andy Fluor™ 405L 412/500 405 nm laser BD Horizon V500 Highly fluorescent; Less spill-over in the green channel
Andy Fluor™ 488 495/520 488 nm laser Alexa Fluor 488, DyLight 488, Cy2, FITC, FAM Extremely photostable; Highly fluorescent and water-soluble
Andy Fluor™ 546 556/575 532, 543, 546, or 555 nm laser Alexa Fluor 546, Cy3, TAMRA Extremely photostable; Highly fluorescent and water-soluble
Andy Fluor™ 555 553/565 532, 543, 546, or 555 nm laser Alexa Fluor 555, DyLight 549, Cy3, TAMRA Brighter than TAMRA and Cy3; Highly photostable and water-soluble
Andy Fluor™ 568 578/602 543, 546, 555, or 568 nm laser Alexa Fluor 568, ATTO 565, Rhodamine Red Highly water-soluble and pH insensitive; Highly fluorescent
Andy Fluor™ 594 590/615 555, or 568 nm laser Alexa Fluor 594, DyLight 594, Texas Red Brighter than Texas Red; Highly water-soluble and pH insensitive
Andy Fluor™ 610 610/625 555, or 568 nm laser Alexa Fluor 610 Highly fluorescent; Highly water-soluble and pH insensitive
Andy Fluor™ 635 630/650 633, or 635 nm laser Alexa Fluor 635, DyLight 633 Highly fluorescent; Highly water-soluble and pH insensitive
Andy Fluor™ 647 650/666 633, or 635 nm laser Alexa Fluor 647, DyLight 649, Cy5 Brighter than Cy5; Highly water-soluble and pH insensitive
Andy Fluor™ 680 680/700 680, or 685 nm laser Alexa Fluor 680, DyLight 680, Cy5.5 Brighter than Alexa Fluor 680; Highly water-soluble and pH insensitive
Andy Fluor™ 750 755/770 680, or 685 nm laser Alexa Fluor 750, DyLight 750, Cy7 Highly fluorescent; Highly water-soluble and pH insensitive 

Brightest Andy Fluor™ Conjugates

Figure 1. Comparison of relative fluorescence of goat anti-rabbit IgG antibody conjugates of Andy Fluor™ dyes and Cy® dyes at different dye:protein ratios in the conjugate.

Figure 2. Flow cytometry comparison of the brightness of Andy Fluor™ goat anti-mouse IgG antibody conjugates with other dye goat anti-mouse IgG antibody conjugates.

Andy Fluor™ Dyes: Excellent Photostability


Figure 3. Comparison of the photobleaching rates of Andy Fluor™ 488 goat anti-mouse IgG antibody conjugate (top) with FITC goat anti-mouse IgG antibody conjugate (bottom).

To order

Buy Cat.No. Product Name Ex/Em(nm) Unit Price
L001 Andy Fluor 350 NHS Ester 350/440 1 µmol $140
L002 Andy Fluor 405M NHS Ester 405/450 1 µmol $220
L003 Andy Fluor 405L NHS Ester 412/500 1 µmol $220
L004 Andy Fluor 488 NHS Ester 495/520 1 µmol $220
L005 Andy Fluor 546 NHS Ester 556/575 1 µmol $220
L006 Andy Fluor 555 NHS Ester 553/565 1 µmol $220
L007 Andy Fluor 568 NHS Ester 578/602 1 µmol $220
L008 Andy Fluor 594 NHS Ester 590/615 1 µmol $220
L009 Andy Fluor 610 NHS Ester 610/625 1 µmol $220
L010 Andy Fluor 635 NHS Ester 630/650 1 µmol $220
L011 Andy Fluor 647 NHS Ester 650/666 1 µmol $220
L012 Andy Fluor 680 NHS Ester 680/700 1 µmol $220
L013 Andy Fluor 750 NHS Ester 755/770 1 µmol $220
L014 Andy Fluor 350 free acid 350/440 5 mg $320
L015 Andy Fluor 405M free acid 405/450 5 mg $320
L016 Andy Fluor 405L free acid 412/500 5 mg $320
L017 Andy Fluor 488 free acid 495/520 5 mg $320
L018 Andy Fluor 546 free acid 556/575 5 mg $320
L019 Andy Fluor 555 free acid 553/565 5 mg $320
L020 Andy Fluor 568 free acid 578/602 5 mg $320
L021 Andy Fluor 594 free acid 590/615 5 mg $320
L022 Andy Fluor 610 free acid 610/625 5 mg $320
L023 Andy Fluor 635 free acid 630/650 5 mg $320
L024 Andy Fluor 647 free acid 650/666 5 mg $320
L025 Andy Fluor 680 free acid 680/700 5 mg $320
L026 Andy Fluor 750 free acid 755/770 5 mg $320
C163 Cy3, free acid 550/570 5 mg $250
C164 Cy3 NHS Ester 550/570 1 mg $180
C167 Cy5, free acid 650/670 5 mg $250
C168 Cy5 NHS Ester 650/670 1 mg $180
C169 Cy5.5, free acid 675/694 5 mg $250
C170 Cy5.5 NHS Ester 675/694 1 mg $180
C171 Cy7, free acid 747/776 5 mg $250
C172 Cy7 NHS Ester 747/776 1 mg $180
C180 Cy3, Bis free acid 550/570 5 mg $250
C181 Cy3 Bis NHS Ester 550/570 1 mg $160
C182 Cy5, Bis free acid 650/670 5 mg $250
C183 Cy5 Bis NHS Ester 650/670 1 mg $160