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LipoFectMax™ Transfection Reagent

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Introduction

LipoFectMax™ Transfection Reagent is a lipid-based transfection reagent that forms a complex with DNA or RNA, and transports the complex into a variety of adherent and suspension cell lines. LipoFectMax™ Transfection Reagent has been tested to work the same efficiency as Lipofectamine® 2000 Reagent, and used for the transfection of both DNA and RNA into eukaryotic cells even in the presence of serum.


Features

  • Superior transfection efficiency for a broad range of cell lines
  • Does not require removal of serum or culture medium
  • Does not require washing or changing of medium after transfection
  • Low cytotoxicity



Figure 1. Assessment of LipoFectMax™ transfection efficiencies using GFP visualization. 8 commonly-used cell lines were transfected with a GFP-expressing plasmid using LipoFectMax™ transfection reagent. Cells were visualized 48 hours post-transfection under fluorescence microscopy (GFP filter).



Figure 2. Comparison of cytotoxicity between LipoFectMax™, Lipofectamine® 2000 and Lipofectamine® 3000.



Figure 3. Assessment of LipoFectMax™ transfection efficiencies for siRNA. Hela cell line was transfected with either a GFP cDNA (Left), or co-tranfected with GFP cDNA and an anti-GFP siRNA (right). Transfection efficiencies were assessed by GFP signal. 



Figure 4. Comparison of LipoFectMax™ and Lipofectamine® 2000 in plasmid transfection on mouse neuronal cells. Mouse neuronal cells were transfected with a GFP-expressing plasmid using either LipoFectMax™ transfection reagent (left), or Lipofectamine® 2000 (right). Cells were visualized 48 hours post-transfection under fluorescence microscopy (GFP filter).

To Order

Buy Cat.No. Product Name Unit Price
FP310 LipoFectMax™ Transfection Reagent 1 mL $200
FP311 LipoFectMax™ Transfection Reagent 3 mL $400

Documents

Protocol (PDF): FP310
MSDS (PDF):  MSDS-FP310
MSDS-FP311