Fluorescent secondary antibodies and streptavidin conjugates are used for the indirect detection of target antigens in many applications including fluorescent cell imaging, western blotting, immunohistochemistry and more. The advantages of using a fluorescently labeled secondary antibody and streptavidin conjugates include brighter signal, multiplexing capabilities, and ease of use. ABP Biosciences offers a wide selection of fluorescent dye conjugated secondary antibodies and streptavidin for your research, featuring our exceptionally bright and photostable Andy Fluor™ dyes. |
Features
- Bright fluorescence with low background
- Excellent photostablility: Resistant to photobleaching
- Great selection of fluorophores: Over 10 different Andy Fluor™ dyes for multicolor imaging
- Instrument compatibility: Excitation and emission spectra fit to the most popular fluorescence instruments’ laser settings and emission filter sets.
Fluorescent Secondary Antibody and Streptavidin Selection Guide
Secondary Antibody | Streptavidin | ||
Andy Fluor™ Dyes | Other Fluorescent Labels | Biotin & HRP Labels | Andy Fluor™ & Cy® Dyes |
Andy Fluor™ 350 | Cy®3 | Biotin | Andy Fluor™ 350 |
Andy Fluor™ 405M | Cy®5 | HRP | Andy Fluor™ 488 |
Andy Fluor™ 430 | Cy®5.5 | Andy Fluor™ 555 | |
Andy Fluor™ 488 | Cy®7 | Andy Fluor™ 594 | |
Andy Fluor™ 555 | FITC | Andy Fluor™ 647 | |
Andy Fluor™ 568 | Cy®3 | ||
Andy Fluor™ 594 | Cy®5 | ||
Andy Fluor™ 610 | |||
Andy Fluor™ 647 | |||
Andy Fluor™ 680 | |||
Andy Fluor™ 750 |
Andy Fluor™ dye conjugates have brighter fluorescence than other fluorophores
Andy Fluor™ dye conjugates have better photostability than other fluorophores
Figure 3. Comparison of the photobleaching rates of Andy Fluor™ 488 goat anti-mouse IgG (H+L) (L109B) with FITC goat anti-mouse IgG (H+L) (L146B). The cytoskeleton of HeLa cells was labeled with mouse monoclonal anti-α-tubulin antibody in combination with Andy Fluor™ 488 goat anti-mouse IgG (H+L) antibody (top series) or with mouse monoclonal anti-α-tubulin antibody in combination with FITC goat anti-mouse IgG (H+L) antibody (bottom series). The fluorescence imaging was taken at 60-second intervals (0, 60, and 120 seconds of exposure).
Labeled Secondary Antibodies
Related Products
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