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Secondary Antibodies and Streptavidin

Introduction

 

Fluorescent secondary antibodies and streptavidin conjugates are used for the indirect detection of target antigens in many applications including fluorescent cell imaging, western blotting, immunohistochemistry and more. The advantages of using a fluorescently labeled secondary antibody and streptavidin conjugates include brighter signal, multiplexing capabilities, and ease of use. ABP Biosciences offers a wide selection of fluorescent dye conjugated secondary antibodies and streptavidin for your research, featuring our exceptionally bright and photostable Andy Fluor™ dyes.

Features

  • Bright fluorescence with low background: Similar performance with Alexa Fluor® conjugates
  • Excellent photostablility: Resistant to photobleaching
  • Great selection of fluorophores: Over 10 different Andy Fluor™ dyes for multicolor imaging
  • Instrument compatibility: Excitation and emission spectra fit to the most popular fluorescence instruments’ laser settings and emission filter sets.

 

Fluorescent Secondary Antibody and Streptavidin Selection Guide

Secondary Antibody Streptavidin
Andy Fluor™ Dyes Other Fluorescent Labels Biotin & HRP Labels Andy Fluor™ & Cy® Dyes
Andy Fluor™ 350 Cy 3 Biotin Andy Fluor™ 350
Andy Fluor™ 405M Cy 5 HRP Andy Fluor™ 488
Andy Fluor™ 430 Cy 5.5   Andy Fluor™ 555
Andy Fluor™ 488 Cy 7   Andy Fluor™ 594
Andy Fluor™ 555 FITC   Andy Fluor™ 647
Andy Fluor™ 568     Cy®3
Andy Fluor™ 594     Cy®5
Andy Fluor™ 610      
Andy Fluor™ 647      
Andy Fluor™ 680      
Andy Fluor™ 750      

Andy Fluor™ dye conjugates have brighter fluorescence than other fluorophores


Figure 1. Comparison of relative fluorescence of goat anti-rabbit IgG antibody conjugates prepared from Andy Fluor™ 488, 555, and 647 with Cy®2, Cy®3, and Cy®5.


Figure 2. Flow cytometry comparison of the brightness of goat anti-mouse IgG antibody conjugates prepared from Andy Fluor™ 488, 555, and 647 with Cy®2, DyLight™ 488, Cy®3, DyLight™ 550, and DyLight™ 650.

Andy Fluor™ dye conjugates have better photostability than other fluorophores



Figure 3. Comparison of the photobleaching rates of Andy Fluor™ 488 goat anti-mouse IgG (H+L) (L109B) with FITC goat anti-mouse IgG (H+L) (L146B). The cytoskeleton of HeLa cells was labeled with mouse monoclonal anti-α-tubulin antibody in combination with Andy Fluor™ 488 goat anti-mouse IgG (H+L) antibody (top series) or with mouse monoclonal anti-α-tubulin antibody in combination with FITC goat anti-mouse IgG (H+L) antibody (bottom series). The fluorescence imaging was taken at 60-second intervals (0, 60, and 120 seconds of exposure).


Figure 4. Immunofluorescent stain of CCSP in mouse lung tissue. FFPE samples of mouse lung were labeled with rabbit anti-CCSP primary antibody, and then visualized with green-fluorescent Andy Fluor™ 488 goat anti-rabbit IgG antibody (green). Nuclei were counterstained with DAPI (blue).


Figure 5. Immunofluorescent stain of CCSP in mouse lung tissue. FFPE samples of mouse lung were labeled with rabbit anti-CCSP primary antibody, and then visualized with either Andy Fluor™ 555 (red, left), or Andy Fluor™ 568 (red, right). Nuclei were counterstained with DAPI (blue).


Figure 6. Immunofluorescent stain of α-tubulin in BEAS2BNNK cells and CCSP in mouse lung tissue. α-Tubulin in fixed and permeabilized BEAS2BNNK cells was labeled with anti-α-tubulin primary antibody, followed by incubation with biotin goat anti-mouse IgG antibody, and then visualized with Andy Fluor™ 488 Streptavidin (green, left). FFPE samples of mouse lung were labeled with rabbit anti-CCSP primary antibody, followed by incubation with biotin goat anti-mouse IgG antibody, and then visualized with Andy Fluor™ 488 Streptavidin (green, middle and right). Nuclei were counterstained with DAPI (blue).

To Order

Andy Fluor™ Labeled Secondary Antibodies

Buy Cat. No. Product Name Ex/Em (nm) Unit Size Price

L100A
L100B
Andy Fluor™ 350 Goat Anti-Mouse IgG (H+L) Antibody 345/440 100 µg
500 µg
$40
$80

L101A
L101B
Andy Fluor™ 350 Goat Anti-Rabbit IgG (H+L) Antibody 345/440 100 µg
500 µg
$40
$80

L103A
L103B
Andy Fluor™ 405M Goat Anti-Mouse IgG (H+L) Antibody 405/450 100 µg
500 µg
$40
$80

L104A
L104B
Andy Fluor™ 405M Goat Anti-Rabbit IgG (H+L) Antibody 405/450 100 µg
500 µg
$40
$80

L106A
L106B
Andy Fluor™ 430 Goat Anti-Mouse IgG (H+L) Antibody 430/540 100 µg
500 µg
$40
$80

L107A
L107B
Andy Fluor 430 Goat Anti-Rabbit IgG (H+L) Antibody 430/540 100 µg
500 µg
$40
$80

L109A
L109B
Andy Fluor™ 488 Goat Anti-Mouse IgG (H+L) Antibody 495/520 100 µg
500 µg
$40
$80

L110A
L110B
Andy Fluor™ 488 Goat Anti-Rabbit IgG (H+L) Antibody 495/520 100 µg
500 µg
$40
$80

L113A
L113B
Andy Fluor™ 555 Goat Anti-Mouse IgG (H+L) Antibody 553/565 100 µg
500 µg
$40
$80

L114A
L114B
Andy Fluor™ 555 Goat Anti-Rabbit IgG (H+L) Antibody 553/565 100 µg
500 µg
$40
$80

L116A
L116B
Andy Fluor™ 568 Goat Anti-Mouse IgG (H+L) Antibody 578/605 100 µg
500 µg
$40
$80

L117A
L117B
Andy Fluor™ 568 Goat Anti-Rabbit IgG (H+L) Antibody 578/605 100 µg
500 µg
$40
$80

L119A
L119B
Andy Fluor™ 594 Goat Anti-Mouse IgG (H+L) Antibody 590/615 100 µg
500 µg
$40
$80

L120A
L120B
Andy Fluor™ 594 Goat Anti-Rabbit IgG (H+L) Antibody 590/615 100 µg
500 µg
$40
$80

L122A
L122B
Andy Fluor™ 610 Goat Anti-Mouse IgG (H+L) Antibody 610/625 100 µg
500 µg
$40
$80

L123A
L123B
Andy Fluor™ 610 Goat Anti-Rabbit IgG (H+L) Antibody 610/625 100 µg
500 µg
$40
$80

L125A
L125B
Andy Fluor™ 647 Goat Anti-Mouse IgG (H+L) Antibody 650/665 100 µg
500 µg
$40
$80

L126A
L126B
Andy Fluor™ 647 Goat Anti-Rabbit IgG (H+L) Antibody 650/665 100 µg
500 µg
$40
$80

L128A
L128B
Andy Fluor™ 680 Goat Anti-Mouse IgG (H+L) Antibody: 680/700 100 µg
500 µg
$40
$80

L129A
L129B
Andy Fluor™ 680 Goat Anti-Rabbit IgG (H+L) Antibody 680/700 100 µg
500 µg
$40
$80

L131A
L131B
Andy Fluor™ 750 Goat Anti-Mouse IgG (H+L) Antibody 750/770 100 µg
500 µg
$40
$80

L132A
L132B
Andy Fluor™ 750 Goat Anti-Rabbit IgG (H+L) Antibody 750/770 100 µg
500 µg
$40
$80

 Cy Labeled Secondary Antibodies

Buy Cat. No. Product Name Ex/Em (nm) Unit Size Price

L134A
L134B
Cy® 3 Goat Anti-Mouse IgG (H+L) Antibody 550/565 100 µg
500 µg
$40
$80

L135A
L135B
Cy® 3 Goat Anti-Rabbit IgG (H+L) Antibody 550/565 100 µg
500 µg
$40
$80

L137A
L137B
Cy® 5 Goat Anti-Mouse IgG (H+L) Antibody 650/667 100 µg
500 µg
$40
$80

L138A
L138B
Cy® 5 Goat Anti-Rabbit IgG (H+L) Antibody 650/667 100 µg
500 µg
$40
$80

L140A
L140B
Cy® 5.5 Goat Anti-Mouse IgG (H+L) Antibody 680/700 100 µg
500 µg
$40
$80

L141A
L141B
Cy® 5.5 Goat Anti-Rabbit IgG (H+L) Antibody 680/700 100 µg
500 µg
$40
$80

L143A
L143B
Cy® 7 Goat Anti-Mouse IgG (H+L) Antibody 750/770 100 µg
500 µg
$40
$80

L144A
L144B
Cy® 7 Goat Anti-Rabbit IgG (H+L) Antibody 750/770 100 µg
500 µg
$40
$80

 FITC Labeled Secondary Antibodies

Buy Cat. No. Product Name Ex/Em (nm) Unit Size Price

L146A
L146B
FITC Goat Anti-Mouse IgG (H+L) Antibody 495/518 100 µg
500 µg
$30
$60

L147A
L147B
FITC Goat Anti-Rabbit IgG (H+L) Antibody 495/518 100 µg
500 µg
$30
$60

 Biotin Labeled Secondary Antibodies

Buy Cat. No. Product Name Ex/Em (nm) Unit Size Price

L149A
L149B
Biotin Goat Anti-Mouse IgG (H+L) Antibody 100 µg
500 µg
$30
$60

L150A
L150B
Biotin Goat Anti-Rabbit IgG (H+L) Antibody 100 µg
500 µg
$30
$60

 HRP Labeled Secondary Antibodies

Buy Cat. No. Product Name Ex/Em (nm) Unit Size Price

L152A
L152B
HRP Goat Anti-Mouse IgG (H+L) Antibody 50 µg
250 µg
$30
$60

L153A
L153B
HRP Goat Anti-Rabbit IgG (H+L) Antibody 50 µg
250 µg
$30
$60

 Streptavidin Conjugates

Buy Cat. No. Product Name Ex/Em (nm) Unit Size Price

L155A
L155B
Andy Fluor™ 350 Streptavidin 345/440 100 µg
500 µg
$40
$80

L156A
L156B
Andy Fluor™ 488 Streptavidin 495/520 100 µg
500 µg
$40
$80

L157A
L157B
Andy Fluor™ 555 Streptavidin 553/565 100 µg
500 µg
$40
$80

L158A
L158B
Andy Fluor™ 594 Streptavidin 590/615 100 µg
500 µg
$40
$80

L159A
L159B
Andy Fluor™ 647 Streptavidin 650/665 100 µg
500 µg
$40
$80

L160A
L160B
Cy®3 Streptavidin 555/568 100 µg
500 µg
$40
$80

L161A
L161B
Cy®5 Streptavidin 650/665 100 µg
500 µg
$40
$80