HRP Goat Anti-Rabbit IgG (H+L) Antibody is prepared from antibodies that have been affinity purified. HRP Goat Anti-Rabbit IgG (H+L) Antibody should be suitable for western blot, ELISA, immunohistochemistry and other standard immunoassay applications. The sensitivity of each lot of antibody is confirmed using ELISA. The specificity of each lot of antibody is confirmed by isoelectric focusing (IEF).
Reconstitution and Storage: Store lyophilized power at 2-8ºC. When ready to use, rehydrate with dH2O (50 µL for 100 µg antibody or 250 µL for 500 µg antibody) to make 2 mg/mL solution and centrifuge if not clear. Product is stable for about 6 months at 2-8ºC as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol for a final concentration of 50%, and store at -20 ºC as a liquid.
| Specifications | Form Information | |||||
| Host/Isotype: | Goat | Physical State: | Lyophilized power | |||
| Class: | Polyclonal | Shipping Condition: | Ambient | |||
| Type: | Secondary Antibody | Purification: | Immunoaffinity chromatography | |||
| Tested Species Reactivity: | Rabbit (Rb) | Buffer: | PBS, pH 7.6, with 1% BSA | |||
| Target Class: | IgG (H+L) | Preservative: | None | |||
| Antibody Form: | Whole Antibody | Storage Conditions: | 4ºC; store in dark | |||
| Label: | HRP | |||||
| Excitation/Emission: | — |
Western blot detection with HRP Goat Anti-Rabbit IgG (H+L) Antibody |
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| Unit Size: | 100 µg, 500 µg | |||||
| Applications | Dilution | |||||
| Western Blot (WB) | 1:10,000 – 1:200,000 | |||||
| Immunocytochemistry (ICC) | 1:500 – 1:5,000 | |||||
| Immunohistochemistry (IHC) | 1:500 – 1:5,000 | |||||
| ELISA | 1:5,000 – 1:100,000 | |||||
