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5-Aminoallyl-dUTP (AA-dUTP)

Introduction

5-Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) (cat. no. C401) is supplied as a 10 mM solution in TE buffer.

5-Aminoallyl-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA polymerase, phi29 DNA Polymerase, Klenow Fragment, Klenow Fragment, exo- and DNA Polymerase I. The resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten. This two-step method for labeling nucleic acids is considerably more economical than the one-step method using a prelabeled dUTP.
 

Specifications:

Concentration: 10 mM solution in TE buffer
Storage: Store at -20°C
Molecular Formula: C12H17N3Na3O14P3
Molecular Weight: 589.17

 

Applications

Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random-primed labeling, or primer extension.

 

To order

Buy Catalog # Product Name Ex/Em (nm) Unit Size List Price
C401 5-Aminoallyl-dUTP, 10 mM in TE buffer 100 µL $120


 

Document

 

Protocol (PDF): C401  
MSDS (PDF):  MSDS-C401