5-Aminoallyl-UTP (5-[3-aminoallyl]-2'-uridine-5'-triphosphate) (cat. no. C402) is supplied as a 10 mM solution in water.
5-Aminoallyl-UTP can be enzymatically incorporated into RNA with T7, T3 and SP6 RNA polymerases. The resulting amine-containing RNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin, or hapten. This two-step method for labeling nucleic acids is considerably more economical than the one-step method using a prelabeled UTP.
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Specifications:
Concentration: 10 mM solution in water
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Applications
Indirect non-radioactive enzymatic labeling of RNA during in vitro transcription.
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