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5-Aminoallyl-UTP (AA-UTP)

Introduction

5-Aminoallyl-UTP (5-[3-aminoallyl]-2'-uridine-5'-triphosphate) (cat. no. C402) is supplied as a 10 mM solution in TE buffer.

5-Aminoallyl-UTP can be enzymatically incorporated into RNA with T7, T3 and SP6 RNA polymerases. The resulting amine-containing RNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin, or hapten. This two-step method for labeling nucleic acids is considerably more economical than the one-step method using a prelabeled UTP.
 

Specifications:

Concentration: 10 mM solution in TE buffer
Storage: Store at -20°C
Molecular Formula: C12H17N3Na3O15P3
Molecular Weight: 605.17

 

Applications

Indirect non-radioactive enzymatic labeling of RNA during in vitro transcription.

 

 

To order

Buy Catalog # Product Name Ex/Em (nm) Unit Size List Price
C402 5-Aminoallyl-UTP, 10 mM in TE buffer 100 µL $110

Document

Protocol (PDF): C402  
MSDS (PDF):  MSDS-C402