The iQuant™ NGS-HS dsDNA Assay Kit is designed specifically for an easy and accurate quantitation of dsDNA. The kit is highly selective for dsDNA over RNA, and offers advantages in stability, linear dynamic range, and sensitivity over other traditional of DNA quantitation. The kit is designed in an "Add-and-Read" format to simplify DNA quantitation and speed up your workflow. The kit is ideal for DNA quantitation in NGS library. The assay is well tolerated to common contaminants such as proteins, salts, solvents, detergents, or free nucleotides. The assay can be adapted for use in microplates, tubes or cuvettes.
Figure 1. Left: Quantitation of dsDNA with iQuant™ NGS-HS dsDNA Assay Kit using fluorescence plate reader. Right: Quantitation of dsDNA with iQuant™ NGS-HS dsDNA Assay Kit using Qubit® Fluorometer.
|Platform:||Fluorescence Plate Reader or Fluorometer|
|Linear Range:||0.2-100 ng dsDNA|
dsDNA quatitation in solution.
Figure 2. iQuant™ NGS-HS dsDNA assay workflow