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iQuant™ dsDNA HS Assay Kit

Introduction

The iQuant™ dsDNA HS Assay Kit is designed specifically for an easy and accurate quantitation of dsDNA. The kit is highly selective for dsDNA over RNA, and offers advantages in stability, linear dynamic range, and sensitivity over other traditional methods of DNA quantitation. The kit contains concentrated quantitation reagent, dilution buffer, and pre-diluted dsDNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the fluorescence using a fluorescence plate reader or fluorometer (Figure 1). The assay is well tolerated to common contaminants such as proteins, salts, solvents, detergents, or free nucleotides. The assay can be adapted for use in microplates, tubes or cuvettes.

Figure 1. Left: Quantitation of dsDNA with iQuant™ dsDNA HS Assay Kit using fluorescence plate reader. Right: Quantitation of dsDNA with iQuant™ dsDNA HS Assay Kit using Qubit® Fluorometer.

 

Specifications:

Sample Type: dsDNA
Platform: Fluorescence plate reader or fluorometer
Linear Range: 0.2-100 ng dsDNA

 

Applications

dsDNA quatitation in solution (Figure 2).

Figure 2. iQuant™ dsDNA HS assay workflow

To Order

Buy Cat. No. Product Name Sensitivity Unit Price
N010 iQuant™ dsDNA HS Assay Kit 0.2-100 ng 200 assays $45
N011 1,000 assays $180

Documents

Protocol (PDF): N010 N011
MSDS (PDF):  MSDS-N010 MSDS-N011
COA (PDF): COA-N010 COA-N011