The iQuant™ dsDNA HS Assay Kit is designed specifically for an easy and accurate quantitation of dsDNA. The kit is highly selective for dsDNA over RNA, and offers advantages in stability, linear dynamic range, and sensitivity over other traditional methods of DNA quantitation. The kit contains concentrated quantitation reagent, dilution buffer, and pre-diluted dsDNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the fluorescence using a fluorescence plate reader or fluorometer (Figure 1). The assay is well tolerated to common contaminants such as proteins, salts, solvents, detergents, or free nucleotides. The assay can be adapted for use in microplates, tubes or cuvettes.
Figure 1. Left: Quantitation of dsDNA with iQuant™ dsDNA HS Assay Kit using fluorescence plate reader. Right: Quantitation of dsDNA with iQuant™ dsDNA HS Assay Kit using Qubit® Fluorometer.
|Platform:||Fluorescence plate reader or fluorometer|
|Linear Range:||0.2-100 ng dsDNA|
dsDNA quatitation in solution (Figure 2).
Figure 2. iQuant™ dsDNA HS assay workflow