The iQuant™ ssDNA Quantitation Kit is ideal for quantitating ssDNA or oligonucleotides. The kit is not selective for ssDNA over dsDNA or RNA, but it will not detect contaminating protein or nucleotides. The kit offers advantages in stability, linear dynamic range, and sensitivity over other traditional of DNA quantitation. The kit contains concentrated quantitation reagent, dilution buffer, and pre-diluted ssDNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the fluorescence using fluorescence plate reader or Fluorometer (Figure 1). The assay is well tolerated to common contaminants such as proteins, salts, solvents, detergents, or free nucleotides. The assay can be adapted for use in microplates, tubes or cuvettes.
Figure 1. Left: Quantitation of ssDNA with iQuant™ ssDNA Assay Kit using fluorescence plate reader. Right: Quantitation of ssDNA with iQuant™ ssDNA Assay Kit using Qubit® Fluorometer.
|Platform:||Fluorescence plate reader or fluorometer|
|Linear Range:||1-200 ng ssDNA|
ssDNA quatitation in solution (Figure 2).
Figure 2. iQuant™ ssDNA assay workflow