Home » Nucleic Acid Quantitation » iQuant™ ssDNA Assay Kit

 

iQuant™ ssDNA Assay Kit

Introduction

The iQuant™ ssDNA Quantitation Kit is ideal for quantitating ssDNA or oligonucleotides. The kit is not selective for ssDNA over dsDNA or RNA, but it will not detect contaminating protein or nucleotides. The kit offers advantages in stability, linear dynamic range, and sensitivity over other traditional of DNA quantitation. The kit contains concentrated quantitation reagent, dilution buffer, and pre-diluted ssDNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the fluorescence using fluorescence plate reader or Fluorometer (Figure 1). The assay is well tolerated to common contaminants such as proteins, salts, solvents, detergents, or free nucleotides. The assay can be adapted for use in microplates, tubes or cuvettes.

Figure 1. Left: Quantitation of ssDNA with iQuant™ ssDNA Assay Kit using fluorescence plate reader. Right: Quantitation of ssDNA with iQuant™ ssDNA Assay Kit using Qubit® Fluorometer.

Specifications:

Sample Type: ssDNA
Platform: Fluorescence plate reader or fluorometer
Linear Range: 1-200 ng ssDNA

 

Applications

ssDNA quatitation in solution (Figure 2).

Figure 2. iQuant™ ssDNA assay workflow

To Order

Buy Cat. No. Product Name Sensitivity Unit Size Price
N014 iQuant™ ssDNA Assay Kit 1-200 ng 200 assays $45
N015 1,000 assays $180

 

 
 
 

Documents

Protocol (PDF): N014 N015
MSDS (PDF):  MSDS-N014 MSDS-N015
COA (PDF): COA-N014 COA-N015