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iQuant™ dsDNA BR Assay Kit

Introduction

The iQuant™ dsDNA BR Assay Kit is designed specifically for an easy and accurate quantitation of dsDNA. The kit is highly selective for dsDNA over RNA, and offers advantages in stability, linear dynamic range, and sensitivity over other traditional of DNA quantitation. The kit contains concentrated quantitation reagent, dilution buffer, and pre-diluted dsDNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the fluorescence using fluorescence plate reader or Fluorometer (Figure 1). The assay is well tolerated to common contaminants such as proteins, salts, solvents, detergents, or free nucleotides. The assay can be adapted for use in microplates, tubes or cuvettes.

Figure 1. Left: Quantitation of dsDNA with iQuant™ dsDNA BR Assay Kit using fluorescence plate reader. Right: Quantitation of dsDNA with iQuant™ dsDNA BR Assay Kit using Qubit® Fluorometer.

 

Specifications:

Sample Type: dsDNA
Platform: Fluorescence plate reader or fluorometer
Linear Range: 2-1000 ng dsDNA

 

Applications

dsDNA quatitation in solution (Figure 2).

Figure 2. iQuant™ dsDNA BR assay workflow

To Order

Buy Cat. No. Product Name Sensitivity Unit Size Price
N012 iQuant™ dsDNA BR Assay Kit 2-1000 ng 200 assays $45
N013 1,000 assays $180

 
 
 

Documents

Protocol (PDF): N012 N013
MSDS (PDF):  MSDS-N012 MSDS-N013
COA (PDF): COA-N012 COA-N013