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iQuant™ RNA HS Assay Kit

Introduction

The iQuant™ RNA HS Assay Kit is designed specifically for an easy and accurate quantitation of RNA. The kit is highly selective for RNA in the present of DNA, and offers advantages in stability, linear dynamic range, and sensitivity over other traditional of RNA quantitation. The kit contains concentrated quantitation reagent, dilution buffer, and pre-diluted RNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the fluorescence using fluorescence plate reader or Fluorometer (Figure 1). The assay is well tolerated to common contaminants such as proteins, salts, solvents, detergents, or free nucleotides. The assay can be adapted for use in microplates, tubes or cuvettes.


Figure 1. Left: Quantitation of ribosomal RNA with iQuant™ RNA HS Assay Kit using fluorescence plate reader. Right: Quantitation of ribosomal RNA with iQuant™ RNA HS Assay Kit using Qubit® Fluorometer.

 

Specifications:

Sample Type: RNA
Platform: Fluorescence plate reader or fluorometer
Linear Range: 5-100 ng RNA

 

Applications

RNA quatitation in solution (Figure 2).


Figure 2. iQuant™ RNA HS assay workflow

To Order

Buy Cat. No. Product Name Sensitivity Unit Size Price
N016 iQuant™ RNA HS Assay Kit 5-100 ng 200 assays $45
N017 1,000 assays $180

   

Documents

Protocol (PDF): N016 N017
MSDS (PDF):  MSDS-N016 MSDS-N017
COA (PDF): COA-N016 COA-N017