2×SYBR Green qPCR Master Mix

ABP SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. The master mix includes HotStart Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I allows for DNA detection and analysis without using sequence-specific probes.

HotStart Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. The SYBR Green qPCR Master Mix provides reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. The SYBR Green qPCR Master Mix is compatible with most real-time thermal cyclers.

SPECIAL FEATURES

• Specificity: HotStart Taq DNA polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers.
• Sensitivity: Detects low copy number targets.
• Wide linear range: Accurate quantification across 9 orders of magnitude.
• Reproducibility and convenience: Ready-to-use 2× master mix.

APPLICATIONS

• Gene-expression analysis.
• siRNA validation.
• Genotyping.
• Pathogen detection.