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iScript™ IV SYBR Green One-Step RT-qPCR Kit

Introduction

iScript™ IV SYBR Green One-Step RT-qPCR Kit is a complete qPCR system containing all necessary reagents for both reverse transcription and PCR amplification to occur in a single reaction qPCR reaction tube. RT-qPCR Enzyme Mix (10X) contains iScript™ IV Reverse Transcriptase (RT), Hot-Start Taq DNA Polymerase and RNase Inhibitor for highly sensitive and specific RT-PCR using any RNA template. Our proprietary RT-qPCR Master Mix (5X) contains stabilizers and enhancers that optimize the two reactions in a real-time “single step”. This One-Step RT-qPCR Kit offers the end-users an efficient and reliable alternative to conventional “two-step” sequential RT-qPCR. Gene-specific primers must be used along with this kit.


Applications

  • Gene-expression analysis.
  • Transcription analysis.
  • Gene cloning.
  • Virus detection and quantification.

 

 

To Order
Buy Cat. No. Product Name Unit Size Price
D045-1 iScript™ IV SYBR Green One-Step RT-qPCR Kit 100 rxns $190
D045-2 iScript™ IV SYBR Green One-Step RT-qPCR Kit 200 rxns $340
 

Documents

Protocol (PDF): D045
MSDS (PDF):  MSDS-D045