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SYTO 9 Green Nucleic Acid Stain


SYTO 9 Green Nucleic Acid Stain is a cell-permeant nucleic acid stain that shows a large fluorescence enhancement upon binding nucleic acids. The SYTO 9 dye can be used to stain RNA and DNA in both live and dead eukaryotic cells, as well as in Gram-positive and Gram-negative bacteria. Eukaryotic cells incubated with SYTO 9 dye generally show cytoplasmic or mitochondrial staining, as well as nuclear staining. This dye is provided as 5 mM solution in DMSO.


  • Permeability to virtually all cell membranes, including mammalian cells and bacteria.
  • High molar absorptivity, with extinction coefficients >50,000 cm-1 M-1 at visible absorption maxima.
  • Extremely low intrinsic fluorescence, with quantum yields typically <0.01 when not bound to nucleic acids.
  • Quantum yields that are typically >0.4 when bound to nucleic acids.


Excitation/Emission: RNA 486/501 nm; DNA 485/498 nm
Shipping Condition: Ambient
Storage Conditions: -20ºC, protect from light
Molecular Formula: C28H27IN2O
Molecular Weight: 534.44
Concentration: 5 mM in DMSO


To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price
C080 SYTO 9 Green Nucleic Acid Stain   100 µL $180 



Protocol (PDF): C080
COA (PDF):  C080