SYTO 9 Green Nucleic Acid Stain is a cell-permeant nucleic acid stain that shows a large fluorescence enhancement upon binding nucleic acids. The SYTO 9 dye can be used to stain RNA and DNA in both live and dead eukaryotic cells, as well as in Gram-positive and Gram-negative bacteria. Eukaryotic cells incubated with SYTO 9 dye generally show cytoplasmic or mitochondrial staining, as well as nuclear staining. This dye is provided as 5 mM solution in DMSO.
Features:
- Permeability to virtually all cell membranes, including mammalian cells and bacteria.
- High molar absorptivity, with extinction coefficients >50,000 cm-1 M-1 at visible absorption maxima.
- Extremely low intrinsic fluorescence, with quantum yields typically <0.01 when not bound to nucleic acids.
- Quantum yields that are typically >0.4 when bound to nucleic acids.
Specifications: | |
Excitation/Emission: | RNA 486/501 nm; DNA 485/498 nm |
Shipping Condition: | Ambient |
Storage Conditions: | -20ºC, protect from light |
Molecular Formula: | C28H27IN2O |
Molecular Weight: | 534.44 |
Concentration: | 5 mM in DMSO |
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