NucTracer 9 Green Nuclear Stain

NucTracer 9 Green Nuclear Stain (Known as SYTO 9 Green Nucleic Acid Stain, Trademark of Thermo Fisher Scientific) is a cell-permeant nucleic acid stain that shows a large fluorescence enhancement upon binding nucleic acids. The NucTracer 9 dye can be used to stain RNA and DNA in both live and dead eukaryotic cells, as well as in Gram-positive and Gram-negative bacteria. Eukaryotic cells incubated with NucTracer 9 dye generally show cytoplasmic or mitochondrial staining, as well as nuclear staining. This dye is provided as 5 mM solution in DMSO.

Features:
 

• Permeability to virtually all cell membranes, including mammalian cells and bacteria.
• High molar absorptivity, with extinction coefficients >50,000 cm^-1 M^-1 at visible absorption maxima.
• Extremely low intrinsic fluorescence, with quantum yields typically <0.01 when not bound to nucleic acids.
• Quantum yields that are typically >0.4 when bound to nucleic acids.