MitoOrange™ CMTMRos (Known as MitoTracker® Orange CMTMRos, Trade Mark of Molecular Probes) is an orange-fluorescent dye that stains mitochondria in live cells and its accumulation is dependent upon membrane potential. The dye is well-retained after aldehyde fixation. This dye is provided as 1 mg/mL solution in DMSO.
|Storage Conditions:||-20ºC, protect from light|
Proc Natl Acad Sci U S A (2012) 109:13978-13983
J Biol Chem (2001) 276:5085-5092
Frequently asked questions (FAQs)
It looks like my Mitotracker™ dye is staining more than just the mitochondria. Why?
This is typically a result of using too high of a concentration of the MitoViewr™ dye. Most organic dyes are used in the low micromolar range. The MitoView™ dyes are used at a much lower concentration, around 50–200 nanomolar. Higher concentrations can cause background fluorescence and non-mitochondrial staining.