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MitoSOX Red


The MitoSOX Red Indicator is a fluorogenic dye for the highly selective detection of superoxide in the mitochondria of live cells. The MitoSOX Red reagent is live-cell permeant which rapidly and selectively target the mitochondria. Once in the mitochondria, the MitoSOX Red reagent is oxidized by superoxide and exhibit bright red fluorescence. The production of superoxide by mitochondria can be visualized in fluorescence microscopy. The MitoSOX Red reagent is readily oxidized by superoxide but not by other reactive oxygen species (ROS) and reactive nitrogen species (RNS).


  • Use for live cell imaging
  • Rapidly and selectively targeted to the mitochondria
  • Readily oxidized by superoxide but not by other ROS or RNS
  • Absorption/emission maxima of 396/610 nm

To order

Buy Cat.No. Product Name Ex/Em(nm) Unit Price
C259-1 MitoSOX Red 396/610 50 µg $65
C259-2 MitoSOX Red 396/610 10×50 µg $295


Datasheet (PDF): C259


Selective fluorescent imaging of superoxide in vivo using ethidium-based probes.
Robinson, Kristine M., et al.
Proceedings of the National Academy of Sciences 103.41 (2006): 15038-15043.

High-content fluorescence imaging with the metabolic flux assay reveals insights into mitochondrial properties and functions.
Little, Andrew Charles, et al.
Communications biology 3.1 (2020): 1-10.

MitoSOX-Based Flow Cytometry for Detecting Mitochondrial ROS.
Kauffman, Megan E. et al.
Reactive oxygen species (Apex, N.C.) vol. 2,5 (2016): 361-370.

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