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DHR 123 [Dihydrorhodamine 123]


Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. Dihydrorhodamine 123 itself is non-fluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. Dihydrorhodamine 123 is useful for detecting reactive oxygen species including superoxide and peroxynitrite. 

Excitation/Emission: 505/534 (oxidized form)
Shipping Condition: Ice pack
Storage Conditions: -20ºC, protect from light
Molecular Formula: C20H18N2O3
Molecular Weight: 346.38
CAS Number: 109244-58-8   


To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price
C261 DHR 123 [Dihydrorhodamine 123]  10 mg $165



Protocol (PDF): C261
COA (PDF):  COA-C261


Superoxide reacts with nitric oxide to nitrate tyrosine at physiological pH via peroxynitrite.
Reiter CD, Teng RJ, Beckman JS
J Biol Chem (2000) 275:32460-32466

Reaction of superoxide and nitric oxide with peroxynitrite. Implications for peroxynitrite-mediated oxidation reactions in vivo.
Jourd'heuil D, Jourd'heuil FL, Kutchukian PS, Musah RA, Wink DA, Grisham MB
J Biol Chem (2001) 276:28799-28805

Modulation of superoxide-dependent oxidation and hydroxylation reactions by nitric oxide.
Miles AM, Bohle DS, Glassbrenner PA, Hansert B, Wink DA, Grisham MB
J Biol Chem (1996) 271:40-47