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JC-1 Dye


JC-1 Dye (5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine chloride) is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. JC-1 exists as a monomer at low concentrations and yields green fluorescence, similar to fluorescein. At higher concentrations, the dye forms J-aggregates that exhibit a broad excitation spectrum and an emission maximum at ~590 nm. These characteristics make JC-1 a sensitive marker for mitochondrial membrane potential. JC-1 is particularly useful for apoptosis studies. In apoptotic cells, the dye stays in the cytoplasm and fluoresces green.

Excitation/Emission: 514/529 nm; 585/590 nm
Shipping Condition: Ambient
Storage Conditions: 2-8ºC, protect from light
Molecular Formula: C25H27Cl4IN4
Molecular Weight: 652.23
CAS Number: 47729-63-5  


To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price
C045 JC-1 Dye 514/529; 585/590 5 mg $195



Protocol (PDF): C045
COA (PDF):  C045 


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