Direct PCR Genotyping Kit

Direct PCR Genotyping Kit is specially designed for the rapid genotyping of mouse, which contains a complete set of reagents for DNA extraction and PCR amplification. This kit can be used for the rapid extraction of genomic DNA from mouse tails, ears, toes, and other tissues. The extracted genomic DNA can be used directly as template for PCR amplification with no need of homogenization, crushing, overnight digestion, phenol-chloroform extraction, DNA precipitation or column purification operations, which greatly shortens the experimental time. During use, immerse the tissue into a lysis buffer, and incubate at 95℃ for 20 min, then add a stop solution to end the extraction. After centrifugation, the lysate can be directly used as the template for PCR amplification. After repeated tests, it is widely applicable to the amplification of target fragments within 2 kb, and is suitable for Multiplex PCR within four fragments.

SPECIAL FEATURES

• Direct PCR: No need for DNA purification steps; crude samples can be added directly to PCR
• Convenient operation: Master Mix format with gel loading dye; reduces sample handling and allows direct loading on gel
• Broad compatibility: Suitable for ≤ 2kb fragments; up to 4-plex amplification

APPLICATIONS

• Mouse Genotyping
• Transgenic mice detection
• Knockout mouse analysis