Introduction
Acridine orange is a cell-permeant nucleic acid binding dye that emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. This unique characteristic makes acridine orange useful for cell-cycle studies. Acridine orange has also been used as a lysosomal dye. Acridine orange is supplied as 10 mg/mL solution in water.
Specifications:
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Excitation/Emission:
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500/526 nm (DNA); 460/650 nm (RNA)
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Shipping Condition:
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Ambient
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Storage Conditions:
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2-8ºC, protect from light
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Molecular Formula:
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C17H20ClN3
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Molecular Weight:
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301.82
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CAS Number:
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65-61-2
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Documents
Reference:
Computer-Assisted Laser Scanning and Video Microscopy for Analysis of Cryptosporidium parvum Oocysts in Soil, Sediment, and Feces.
Anguish LJ, Ghiorse WC
Appl Environ Microbiol (1997) 63:724-724Product usage:
Fuller ME, Streger SH, Rothmel RK, Mailloux BJ, Hall JA, Onstott TC, Fredrickson JK, Balkwill DL, DeFlaun MF
Appl Environ Microbiol (2000) 66:4486-4496
Product usage:
Leys N, Baatout S, Rosier C, Dams A, s'Heeren C, Wattiez R, Mergeay M,
Antonie Van Leeuwenhoek (2009) 96:227-245