Monobromobimane (mBBr) is essentially nonfluorescent, and freely passively diffuse across the plasma membrane into the cytoplasm, where they form blue-fluorescent adducts with intracellular glutathione and thiol containing proteins. The glutathione conjugate of monobromobimane has absorption/emission maxima ~394/490 nm. mBBr has been reported to be used as effective tracer of gap-junctional communication.
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Specifications: |
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Excitation/Emission: |
385/463 nm |
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Shipping Condition: |
Ambient |
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Storage Conditions: |
-20ºC, protect from light | |
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Molecular Formula: |
C10H11BrN2O2 |
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Molecular Weight: |
271.11 |
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CAS Number: |
71418-44-5 |
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| Protocol (PDF): | C038 |
| MSDS (PDF): | MSDS-C038 |
| COA (PDF): | COA-C038 |
Reference:
J Biol Chem (1998) 273:30255-30262
Biochemistry (1988) 27:4512-4522
J Muscle Res Cell Motil (1992) 13:132-145
