BCECF is an ideal dual-excitation ratiometric pH indicator for measuring changes ± 1 pH unit around pH 7 with a pKa of ~6.98. pH measurements with BCECF are made by determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at ~490 nm versus the emission intensity when excited at its isobestic point of ~440 nm.

Excitation/Emission: 490/535
Shipping Condition: Ambient
Storage Conditions: 2-8ºC, protect from light
Molecular Formula: C27H20O11
Molecular Weight: 520.45
CAS Number: 85138-49-4  


To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price
C027 BCECF 490/535 5 mg $150




Protocol (PDF): C027
MSDS (PDF):  C027

Fluorescent indicators for intracellular pH.
Han J, Burgess K,
Chem Rev (2010) 110:2709-2728

Wavelength Considerations in Confocal Microscopy of Botanical Specimens.
Fricker MD, White NS
J Microsc (1992) 166:29-29

Calibration Methods and Avoidance of Errors in Measurement of Intracellular pH (pHcyt) Using the Indicator Bis (Carboxyethyl)-5(6)-Carboxyfluorescein (BCECF) in Human Platelets.
Valant PA, Haynes DH
J Fluorescence (1992) 2:191-191