5(6)-CFDA is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Upon hydrolysis by intracellular esterases, the 5(6)-carboxyfluorescein diacetate yields 5(6)-carboxyfluorescein. As compared with fluorescein, carboxyfluorescein contains extra negative charges and is therefore better retained in cells.
Specifications: | ||
Excitation/Emission: | 490/520 (after cleavage) | |
Shipping Condition: | Ambient | |
Storage Conditions: | -20ºC, protect from light | |
Molecular Formula: | C25H16O9 | |
Molecular Weight: | 460.40 | |
CAS Number: | 124387-19-5 | |
Protocol (PDF): | C031 |
MSDS (PDF): | MSDS-C031 |
COA (PDF): | C031 |
References:
Breeuwer P, et al.
Appl Environ Microbiol (1997) 63:178-178
Proc SPIE-Int Soc Opt Eng (1999) 3755:24-32
Immunol Cell Biol (1999) 77:499-508