5(6)-CFDA is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Upon hydrolysis by intracellular esterases, the 5(6)-carboxyfluorescein diacetate yields 5(6)-carboxyfluorescein. As compared with fluorescein, carboxyfluorescein contains extra negative charges and is therefore better retained in cells.

Excitation/Emission: 490/520 (after cleavage)
Shipping Condition: Ambient
Storage Conditions: -20ºC, protect from light
Molecular Formula: C25H16O9
Molecular Weight: 460.40
CAS Number: 124387-19-5  


To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price
C031 CFDA 490/520 100 mg $110



Protocol (PDF): C031
COA (PDF): C031


Nonanoic Acid, a Fungal Self-Inhibitor, Prevents Germination of Rhizopus oligosporus Sporangiospores by Dissipation of the pH Gradient.
Breeuwer P, et al.
Appl Environ Microbiol (1997) 63:178-178

Fluorescent microscopic system for detection of microorganisms and organaic compounds on Mars.
Saito T, Kawasaki Y, Kaneko T, Kobayashi K, Miyakawa A
Proc SPIE-Int Soc Opt Eng (1999) 3755:24-32

Fluorescent dyes for lymphocyte migration and proliferation studies.
Parish CR
Immunol Cell Biol (1999) 77:499-508