5(6)-CDCFDA is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Upon hydrolysis by intracellular esterases, the 5(6)-CDCFDA yields the fluorescent fluorophore, 5-(and-6)-carboxy-2',7' -dichlorofluorescein. With a pKa of 4.8, this dye can be used as an acidic pH sensor.
|Excitation/Emission:||504/529 nm (after cleavage)|
|Storage Conditions:||-20ºC, protect from light|