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EasyProbe™ Propidium Iodide Dead Cell Stain

Introduction

EasyProbes™ Propidium iodide is a commonly used dead-cell stain that emits red fluorescence upon binding to DNA. Our EasyProbes™ Propidium Iodide Dead Cell Stain is formulated in a ready-to-use solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. Propidium iodide is membrane-impermeant and excluded from viable cells. It is therefore commonly used for identifying dead cells in a population, and for counting dead cells, measuring apoptosis, and sorting cells based on DNA content.

Features

  • No need to dilute, weigh, or pipette
  • Convenient dropper bottle—just use two drops per mL
  • Rapid staining of dead cells without wash steps


Suggestions for use

  • In most cases 2 drops/1×106 cells and an incubation of 15 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases simply add more, or fewer drops until the optimal staining intensity is obtained.
  • EasyProbes™ Propidium Iodide Dead Cell Stain is excited with a xenon or mercury-arc lamp or with the 488 line of an argon-ion laser when bound to DNA, with an emission maximum at 617 nm. Generally, propidium iodide fluorescence is detected in the FL2 channel of flow cytometers.
Specifications:  
Excitation/Emission: 535/617 nm
Shipping Condition: Ambient
Storage Conditions: 4ºC, protect from light
Unit Size: 10 mL

 

To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price
FP028 EasyProbe™ Propidium Iodide Dead Cell Stain 535/617 10 mL $60 

Documents

Protocol (PDF): FP028
MSDS (PDF):  FP028
COA (PDF):  FP028

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