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EMA (Ethidium monoazide)

Introduction

Ethidium monoazide is a fluorescent photoafinity label nucleic acid stain, after photolysis, which binds covalently to nucleic acids both in solution and in cells that have compromised membranes. Ethidium monoazide bromide is relatively impermeant to live cells, it selectively labels DNA in dead cells in a mixed population of live and dead cells. The fluorescence of ethidium monoazide is weak, but the intensity increases ~15-fold upon binding to DNA with excitation/emission maxima of ~504/600 nm.

Specifications:  
Excitation/Emission: 504/600 nm
Shipping Condition: Ambient
Storage Conditions: -20ºC, protect from light
Molecular Formula: C21H18BrN5
Molecular Weight: 420.31
CAS Number: 58880-05-0   
     
             

 

To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price
C009 EMA (Ethidium monoazide) 504/600 5 mg $120

Documents

Protocol (PDF): C009
MSDS (PDF):  MSDS-C009
COA (PDF):  C009 

Reference:

Analysis of HCV-specific T cells by flow cytometry.
Shiina M, Rehermann B,
Methods Mol Biol (2009) 510:415-426

Fluorescent Imaging of Cryptosporidium Using a Cooled Charge Couple Device (CCD).
Campbell AT, et al.
J Microbiol Methods (1992) 16:169-169

TLR9 signals after translocating from the ER to CpG DNA in the lysosome.
Latz E, Schoenemeyer A, Visintin A, Fitzgerald KA, Monks BG, Knetter CF, Lien E, Nilsen NJ, Espevik T, Golenbock DT
Nat Immunol (2004) 5:190-198