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EasyProbe™ DAPI Fixed Cell Stain

Introduction

EasyProbes™ DAPI is a commonly used nuclear counterstain for fixed cells that emits blue fluorescence upon binding to the minor groove of dsDNA. Our EasyProbes™ DAPI Fixed Cell Stain is formulated in a ready-to-use solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. DAPI is widely used for nuclear and chromosome counterstain, showing a distinct banding pattern in chromosomes.

Features

  • No need to dilute, weigh, or pipette
  • Convenient dropper bottle—just use two drops per mL
  • Excited by UV light and emits blue fluorescence at 460 nm when bound to DNA

Suggestions for use

  • In most cases 2 drops/ml and an incubation of 15 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases simply add more, or fewer drops until the optimal staining intensity is obtained.
  • EasyProbes™ DAPI Fixed Cell Stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm. It is detected through a blue/cyan filter, such as a DAPI filter set.
Specifications:  
Excitation/Emission: 358/460 nm
Shipping Condition: Ambient
Storage Conditions: 4ºC, protect from light
Unit Size: 10 mL

 

To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price
FP026 EasyProbe™ DAPI Fixed Cell Stain 358/460 10 mL $60

Documents

Protocol (PDF): FP026
MSDS (PDF):  FP026
COA (PDF):  FP026

Reference:

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Chan B, VanderLaan PA, Sukhatme VP, 
Biochem Biophys Res Commun (2013) 439:247-251


A plasmid-based reporter system for live cell imaging of dengue virus infected cells.

Medin CL, Valois S, Patkar CG, Rothman AL, 
J Virol Methods (2015) 211:55-62


An iron-regulated and glycosylation-dependent proteasomal degradation pathway for the plasma membrane metal transporter ZIP14.

Zhao N, Zhang AS, Worthen C, Knutson MD, Enns CA, 
Proc Natl Acad Sci U S A (2014) -111(25):9175-80.