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EasyProbe™ Green 488 Dead Cell Stain

Introduction

EasyProbes™ Green 488 Dead Cell Stain is a cell-impermeant stain that emits exceptionally bright, green fluorescence when bound to DNA. Our EasyProbes™ Green 488 Dead Cell Stain is formulated in a ready-to-use solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. EasyProbes™ Green 488 Dead Cell Stain is a useful stain to estimate live/dead cell ratios and to measure cytotoxicity in kinetic live-cell assays. It is also suitable for staining nuclei in fixed cell preparations and tissue sections.

Features

  • No need to dilute, weigh, or pipette
  • Convenient dropper bottle—just use two drops per mL
  • Rapid staining of damaged, dead, or fixed cells without wash steps

Suggestions for use

EasyProbes™ Green 488 Dead Cell Stain may be added directly to cells in full media or buffer solutions. It easily stains nuclei of fixed cells and tissue slices

  • In most cases 2 drops/ml and an incubation of 15 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases simply add more, or fewer drops until the optimal staining intensity is obtained.
  • EasyProbes™ Green 488 Dead Cell Stain is excited with a maximum at 500 nm when bound to DNA, with an emission maximum at 530 nm. It is detected through standard GFP and FITC filters.
Specifications:  
Excitation/Emission: 500/530 nm
Shipping Condition: Ambient
Storage Conditions: 4ºC, protect from light
Unit Size: 10 mL

 

To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price
FP030 EasyProbe™ Green 488 Dead Cell Stain 500/530 10 mL $60

Documents

Protocol (PDF): FP030
MSDS (PDF):  FP030
   

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