RNAzol RT Reagent | ABP Biosciences

Introduction

RNAzol RT Reagent is a ready-to-use reagent, designed to isolate high quality total RNA and small RNA from samples of human, animal, plant, bacterial and viral origin. RNAzol RT Reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components which facilitate the isolation of a variety of RNA species of large or small molecular size. RNAzol RT Reagent maintains the integrity of the RNA due to highly effective inhibition of RNase activity while disrupting cells and dissolving cell components during sample homogenization. RNAzol RT Reagent allows for simultaneous processing of a large number of samples.

Features

No chloroform-induced phase separation is necessary to obtain pure RNA. Just add water to remove DNA, proteins, polysaccharides and other contaminants.
The isolation procedure can be completed in less than one hour and is performed at room temperature, including all centrifugation steps.
RNAzol RT isolates total RNA, or large RNA and small RNA in separate fractions. The large RNA fraction contains rRNA and mRNA. The small RNA fraction contains tRNA, small RNA and microRNA.
The isolated RNA is ready for RT-PCR, qRT-PCR, microarrays, poly A+ selection, northern blotting, RNase protection assay and other molecular biology applications.

To Order

Documents

Protocol (PDF):	FP314
MSDS (PDF):	MSDS-FP314

Cited Reference:

Identification of Diagnostic Markers Correlated With HIV+ Immune Non-response Based on Bioinformatics Analysis
Bai R, Li Z, Hou Y, Lv S, Wang R, Hua W, Wu H and Dai L
Front. Mol. Biosci. 2021, 8:809085. doi: 10.3389/fmolb.2021.809085

Identification and Validation of Hub Genes Associated with Hepatocellular Carcinoma Via Integrated Bioinformatics Analysis
Wang S, Song Z, Tan B, Zhang J, Zhang J and Liu S
Front Oncol (2020) 10:681. doi: 10.3389/fonc.2020.00681

Exploring the biological function of immune cell-related genes in human immunodeficiency virus (HIV)-1 infection based on weighted gene co-expression network analysis (WGCNA)
Bai, R., Li, Z., Lv, S. et al.
BMC Med Genomics 15, 200 (2022). https://doi.org/10.1186/s12920-022-01357-y

Cell Proliferation and Tumor Induction by Ochratoxin A in Mouse Skin and Evaluation of Cyclin D1 and Cyclooxygenase-2 Expressions
Sai Yang and Yajia Wen
Computational and Mathematical Methods in Medicine, 2021, Article ID 1723486, https://doi.org/10.1155/2021/1723486

Differentially Infiltrated Identification of Novel Diagnostic Biomarkers Associated with Immune Infiltration in Nasopharyngeal Carcinoma
Pei Gao , Wuhao Lu, Shousen Hu, and Kun Zhao
Disease Markers, 2022, Article ID 3934704, https://doi.org/10.1155/2022/3934704

Screening of DNA Damage Repair Genes Involved in the Prognosis of Triple-Negative Breast Cancer Patients Based on Bioinformatics
Wang N, Gu Y, Chi J, Liu X, Xiong Y, Zhong C, Wang F, Wang X and Li L
Front. Genet. 2021, 12:721873. doi: 10.3389/fgene.2021.721873

Identification and validation of HOXD3 and UNC5C as molecular signatures in keloid based on weighted gene co-expression network analysis
Hanwen Wang, Ziheng Zhou, Yiling Liu, Peng Wang, Lei Chen, Shaohai Qi, Julin Xie, Jinming Tang
Genomics, 2022, 114, 4, 110403, https://doi.org/10.1016/j.ygeno.2022.110403

A Coagulation-Related Gene-Based Prognostic Model for Invasive Ductal Carcinoma
Li J, Du J, Wang Y and Jia H
Front. Genet. 2021, 12:722992. doi: 10.3389/fgene.2021.722992

Identification of CALU and PALLD as Potential Biomarkers Associated with Immune Infiltration in Heart Failure.
Liu X, Xu S, Li Y, Chen Q, Zhang Y and Peng L
Front. Cardiovasc. Med. 2021, 8:774755. doi: 10.3389/fcvm.2021.774755

Comparative bioinformatics analysis and abiotic stress responses of expansin proteins in Cucurbitaceae members: watermelon and melon
Incili, Ç.Y., Arslan, B., Çelik, E.N.Y. et al.
Protoplasma (2022). https://doi.org/10.1007/s00709-022-01793-8

Dexmedetomidine Mitigates Myocardial Ischemia/Reperfusion-Induced Mitochondrial Apoptosis through Targeting lncRNA HCP5
Xu Deng, Fei Ye, Lixiong Zeng, Wenzhi Luo, Shan Tu, Xiaoyan Wang and Zhihui Zhang
The American Journal of Chinese Medicine, 2022, 50, 1529-1551. https://doi.org/10.1142/S0192415X22500641

Integrated analysis of differential lncRNA and mRNA expression in cholelithiasis based on high-throughput sequencing and bioinformatics
C Xu, Y Sun, Y Li, Q Xu, W Li, P Xu
Research Square, 2022. DOI: https://doi.org/10.21203/rs.3.rs-1708027/v1

Glycosyltransferases EXTL2 and EXTL3 cellular balance dictates heparan sulfate biosynthesis and shapes gastric cancer cell motility and invasion
Catarina Marques, Juliana Poças, Catarina Gomes, Isabel Faria-Ramos, Celso A. Reis, Romain R. Vivès, and Ana Magalhães
J. Biol. Chem. (2022) 298(11) 102546. https://doi.org/10.1016/j.jbc.2022.102546

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Buy	Cat.No.	Product Name	Unit	Price
	FP314	RNAzol RT Reagent	100 mL	$130