Introduction
MitoTrack™ Green FM (Known as MitoTracker® Green FM, Trade Mark of ThermoFisher Scientific) is an green-fluorescent dye that stains mitochondria in live cells and its accumulation is dependent upon membrane potential. The dye is not well-retained after aldehyde fixation.
Specifications:
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Excitation/Emission:
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490/516 nm
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Shipping Condition:
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Ambient
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Storage Conditions:
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-20ºC, protect from light
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Molecular Formula:
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C34H28Cl5N3O
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Molecular Weight:
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671.88
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CAS Number:
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N/A
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Documents
Reference
Shim SH, Xia C, Zhong G, Babcock HP, Vaughan JC, Huang B, Wang X, Xu C, Bi GQ, Zhuang X,
Proc Natl Acad Sci U S A (2012) 109:13978-13983
Lipscomb EA, Sarmiere PD, Freeman RS
J Biol Chem (2001) 276:5085-5092
Frequently asked questions (FAQs)
It looks like my Mitotracker™ dye is staining more than just the mitochondria. Why?
This is typically a result of using too high of a concentration of the MitoViewr™ dye. Most organic dyes are used in the low micromolar range. The MitoView™ dyes are used at a much lower concentration, around 50–200 nanomolar. Higher concentrations can cause background fluorescence and non-mitochondrial staining.